3-Dimensional Tracking of Non-blinking 'Giant' Quantum Dots in Live Cells

Advanced Functional Materials
Aaron M KellerJames H Werner

Abstract

While semiconductor quantum dots (QDs) have been used successfully in numerous single particle tracking (SPT) studies due to their high photoluminescence efficiency, photostability, and broad palette of emission colors, conventional QDs exhibit fluorescence intermittency or 'blinking,' which causes ambiguity in particle trajectory analysis and limits tracking duration. Here, non-blinking 'giant' quantum dots (gQDs) are exploited to study IgE-FcεRI receptor dynamics in live cells using a confocal-based 3D SPT microscope. There is a 7-fold increase in the probability of observing IgE-FcεRI for longer than 1 min using the gQDs compared to commercially available QDs. A time-gated photon-pair correlation analysis is implemented to verify that selected SPT trajectories are definitively from individual gQDs and not aggregates. The increase in tracking duration for the gQDs allows the observation of multiple changes in diffusion rates of individual IgE-FcεRI receptors occurring on long (>1 min) time scales, which are quantified using a time-dependent diffusion coefficient and hidden Markov modeling. Non-blinking gQDs should become an important tool in future live cell 2D and 3D SPT studies, especially in cases where changes in cellular...Continue Reading

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Citations

Jan 3, 2016·The Review of Scientific Instruments·M S DeVoreJ H Werner
May 13, 2017·Nature Communications·Nimai MishraJennifer A Hollingsworth
Feb 13, 2016·Nanoscale·Aidi ZhangJicun Ren
Sep 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·I V MartynenkoY K Gun'ko
May 29, 2018·Nature Methods·Dayong JinAntoine M van Oijen
Aug 1, 2015·Physical Review Letters·Mehdi SadatgolDurdu Ö Güney
Oct 25, 2017·Nanoscale·Margaret ChernAllison M Dennis
Aug 12, 2015·The Journal of Physical Chemistry Letters·Melissa PauliteHan Htoon
Jan 18, 2020·Chemical Reviews·Shu-Lin LiuDai-Wen Pang

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