PMID: 3745272Sep 1, 1986Paper

3-Hydroxy-3-methylglutaryl coenzyme A reductase localization in rat liver peroxisomes and microsomes of control and cholestyramine-treated animals: quantitative biochemical and immunoelectron microscopical analyses

The Journal of Cell Biology
G A KellerS Krisans

Abstract

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, a key regulatory enzyme involved in cholesterol biosynthesis, has recently been reported to be present in rat liver peroxisomes (Keller, G.A., M.C. Barton, D.J. Shapiro, and S.J. Singer, 1985, Proc. Natl. Acad. Sci. USA, 82:770-774). Immunoelectron labeling of ultrathin frozen sections of normal liver, using two monoclonal antibodies to purified rat liver microsomal HMG-CoA reductase, indicated that the enzyme is present in the matrix of peroxisomes. This study is a quantitative biochemical and immunoelectron microscopical analysis of HMG-CoA reductase in rat liver peroxisomes and microsomes of normal and cholestyramine-treated animals. Cholestyramine treatment produced a six- to sevenfold increase in the specific activity of peroxisomal HMG-CoA reductase, whereas the microsomal HMG-CoA reductase specific activity increased by about twofold. Using a computer program that calculates optimal linear combinations of marker enzymes, it was determined that between 20 and 30% of the total reductase activity was located in the peroxisomes of cholestyramine-treated animals. Less than 5% of the reductase activity was present in peroxisomes under control conditions. Quantitation o...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P B Lazarow, C De Duve
Jun 13, 1979·Biochemical and Biophysical Research Communications·M BronfmanF Leighton
Jul 1, 1985·The Journal of Cell Biology·S E AlexsonP B Lazarow
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G A KellerS J Singer
May 1, 1973·The Journal of Cell Biology·K T Tokuyasu
Dec 29, 1974·Biochimica Et Biophysica Acta·D J ShapiroR T Schimke
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C F ClarkeA M Fogelman
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G A KellerS J Singer
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L LiscumM S Brown
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R E ClarkD J Shapiro
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D J ChinJ L Goldstein
Jan 1, 1982·Annals of the New York Academy of Sciences·A K Hajra, J E Bishop
Aug 1, 1982·Biochemical and Biophysical Research Communications·L R Hagey, S K Krisans

❮ Previous
Next ❯

Citations

Jan 1, 1987·Journal of Inherited Metabolic Disease·P B Lazarow
Jun 15, 1990·Experientia·M P Reinhart
Dec 21, 2006·Histochemistry and Cell Biology·Werner J KovacsSkaidrite K Krisans
Aug 1, 1990·Journal of the Neurological Sciences·R J WandersJ M Tager
Nov 25, 1998·Molecular Aspects of Medicine·R J Wanders, J M Tager
Jan 1, 1988·Experimental Pathology·P RebuffatG G Nussdorfer
Oct 1, 1986·Journal of Ultrastructure and Molecular Structure Research·Y D StierhofH Frank
Dec 31, 2003·Molecular Biology of the Cell·Javier Peña-DiazDolores Gonzalez-Pacanowska
Jan 1, 1995·European Journal of Clinical Investigation·E MalleG M Kostner
Dec 27, 1996·Annals of the New York Academy of Sciences·S K Krisans
Dec 4, 2002·Annual Review of Entomology·Steven J Seybold, Claus Tittiger
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·S K Krisans
Feb 18, 2015·The Journal of Cell Biology·Arnaud MourierNils-Göran Larsson
Aug 24, 1999·FEBS Letters·T P ThaiW W Just
Sep 1, 1992·Cell Biochemistry and Function·G P Mannaerts, P P Van Veldhoven
Sep 11, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Marco Fidaleo
Dec 16, 1991·Biochemical and Biophysical Research Communications·V J HodgeS K Krisans
Nov 1, 1989·Archives of Biochemistry and Biophysics·H Hayashi, A Miwa
Jun 3, 2004·Experimental Parasitology·Wilfredo QuiñonesJuan Luis Concepción
May 9, 2006·Biochemical and Biophysical Research Communications·Isabelle WeinhoferJohannes Berger
Nov 1, 1990·Archives of Biochemistry and Biophysics·E L AppelkvistG Dallner
Apr 10, 2016·Biochimica Et Biophysica Acta·Eduardo Silva RamosArnaud Mourier
Aug 15, 2009·Interdisciplinary Perspectives on Infectious Diseases·Wanderley de Souza, Juliany Cola Fernandes Rodrigues
Dec 20, 2011·Computers in Biology and Medicine·M V K KarthikPratyoosh Shukla
Mar 15, 1997·Microscopy Research and Technique·M M Magalhães, M C Magalhães
Aug 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M LindauerH D Fahimi
Oct 29, 1987·Biochemical and Biophysical Research Communications·N Rusnak, S K Krisans
Nov 28, 2002·Journal of Lipid Research·Rainer Breitling, Skaidrite K Krisans
Aug 17, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N AboushadiS K Krisans
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H D Fahimi, E Baumgart
Dec 1, 2001·Biological & Pharmaceutical Bulletin·A MichiharaH Tsuji

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.