PMID: 9171410Jun 1, 1997Paper

3-hydroxy-3-methylglutaryl coenzyme A reductase of Sulfolobus solfataricus: DNA sequence, phylogeny, expression in Escherichia coli of the hmgA gene, and purification and kinetic characterization of the gene product

Journal of Bacteriology
D A BocharV W Rodwell

Abstract

The gene (hmgA) for 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (EC 1.1.1.34) from the thermophilic archaeon Sulfolobus solfataricus P2 was cloned and sequenced. S. solfataricus HMG-CoA reductase exhibited a high degree of sequence identity (47%) to the HMG-CoA reductase of the halophilic archaeon Haloferax volcanii. Phylogenetic analyses of HMG-CoA reductase protein sequences suggested that the two archaeal genes are distant homologs of eukaryotic genes. The only known bacterial HMG-CoA reductase, a strictly biodegradative enzyme from Pseudomonas mevalonii, is highly diverged from archaeal and eukaryotic HMG-CoA reductases. The S. solfataricus hmgA gene encodes a true biosynthetic HMG-CoA reductase. Expression of hmgA in Escherichia coli generated a protein that both converted HMG-CoA to mevalonate and cross-reacted with antibodies raised against rat liver HMG-CoA reductase. S. solfataricus HMG-CoA reductase was purified in 40% yield to a specific activity of 17.5 microU per mg at 50 degrees C by a sequence of steps that included heat treatment, ion-exchange chromatography, hydrophobic interaction chromatography, and affinity chromatography. The final product was homogeneous, as judged by sodium dodecyl sulfate-p...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C R Woese, G E Fox
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 25, 1992·Nucleic Acids Research·J HainW Zillig
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·K M Bischoff, V W Rodwell
Dec 1, 1992·Trends in Biochemical Sciences·M W SmithR F Doolittle
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 1, 1987·Microbiological Reviews·C R Woese
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G PühlerW Zillig
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P GogartenT Oshima
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N IwabeT Miyata
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W L Lam, W F Doolittle
Nov 25, 1988·Nucleic Acids Research·F Corpet
Mar 1, 1986·Microbiological Reviews·M De RosaA Gliozzi
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Dec 29, 1974·Biochimica Et Biophysica Acta·D J ShapiroR T Schimke
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Oct 20, 1995·Science·C M FraserJ C Venter
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J R Brown, W F Doolittle
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·W F Doolittle, J R Brown
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·T L MarshG J Olsen

❮ Previous
Next ❯

Citations

Feb 28, 2001·Environmental Microbiology·R Fall, S D Copley
Jan 24, 2019·Extremophiles : Life Under Extreme Conditions·Geraldy L S LimanThomas J Santangelo
Dec 22, 2007·Annals of the New York Academy of Sciences·Laura L Grochowski, Robert H White
May 7, 2011·Natural Product Reports·Tohru DairiIsao Fujii
Nov 16, 2017·Chembiochem : a European Journal of Chemical Biology·Michael DirkmannFrank Schulz
May 21, 2004·Protein Science : a Publication of the Protein Society·Matija Hedl, Victor W Rodwell
Feb 16, 2017·The Journal of Antibiotics·Tomohisa Kuzuyama
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Yosuke Koga, Hiroyuki Morii
Mar 9, 1999·Molecular Genetics and Metabolism·D A BocharV W Rodwell
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·J R Brown, W F Doolittle
Jan 29, 2000·Journal of Natural Products·W P WagnerR Fall
Nov 3, 2010·Research in Microbiology·Rie MatsumiJohn van der Oost

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.