3-phosphohistidine and 6-phospholysine are substrates of a 56-kDa inorganic pyrophosphatase from bovine liver

Archives of Biochemistry and Biophysics
H HiraishiA Kumon

Abstract

A 56-kDa inorganic pyrophosphatase isolated from bovine liver hydrolyzed PPi, imidodiphosphate, 3-phosphohistidine, and 6-phospholysine at rates of 0.11, 0.44, 1.09, and 1.22 mumol/min/mg protein, respectively, in a reaction mixture containing 1 mM MgCl2 at pH 8.2. The hydrolysis of imidodiphosphate was influenced by various treatments in a different manner from that of N-phosphorylated amino acids, indicating that the pyrophosphatase has two different catalytic sites for imidodiphosphate and N-phosphorylated amino acids, respectively. Evidence for separate catalytic sites consists of the following findings: the activity on hydrolysis of imidodiphosphate gave a bell-shaped pH curve with a peak at pH 6.5, while the activity on hydrolysis of N-phosphorylated amino acids maintained a high level in the pH range between 6.0 and 9.5. One hundred micromolar p-chloromercuriphenyl sulfonate inhibited the hydrolysis of imidodiphosphate by 35% and did not inhibit that of N-phosphorylated amino acids. Two millimolar magnesium chloride repressed the hydrolysis of imidodiphosphate and had no inhibitory effect on the hydrolysis of N-phosphorylated amino acids. Moreover, methylenediphosphonic acid, an analog of imidodiphosphate, stimulated the...Continue Reading

References

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Citations

Oct 21, 2015·Advances in Biological Regulation·Cristina Azevedo, Adolfo Saiardi
Jan 25, 2006·Biochemical and Biophysical Research Communications·Eisuke KoikeHajime Sugihara
Sep 29, 2005·Biochimica Et Biophysica Acta·Paul G Besant, Paul V Attwood
Feb 11, 2010·Bioscience Reports·Karolina KowalewskaZbigniew Szewczuk
Mar 28, 2012·Biochimica Et Biophysica Acta·Paul V Attwood
May 29, 2015·Organic & Biomolecular Chemistry·Jordi Bertran-VicenteChristian P R Hackenberger
Oct 30, 2018·Chembiochem : a European Journal of Chemical Biology·Hoyoung JungJung-Min Kee
Mar 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Janusz J PetkowskiSara Seager
Dec 5, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mehul V MakwanaRichard Fw Jackson
Mar 22, 2018·Nature·Sravanth K HindupurMichael N Hall
Feb 11, 2016·Biochemical Society Transactions·Cristina Azevedo, Adolfo Saiardi
Jun 28, 2020·Nature Communications·Iain M HayJanet E Deane
Sep 29, 2020·Chemistry : a European Journal·Anett HauserChristian P R Hackenberger
Jan 6, 2021·The International Journal of Biochemistry & Cell Biology·Dan WangZhiqiang Meng
Sep 10, 2014·Journal of the American Chemical Society·Jordi Bertran-VicenteChristian P R Hackenberger
Sep 5, 2021·Rapid Communications in Mass Spectrometry : RCM·Xuelian ZhaoFeng Ni

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