3'-Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

British Journal of Pharmacology
Li-Jung KangSiyoung Yang

Abstract

3'-Sialyllactose (3'-SL) is a safe compound that is present in high levels in human milk. Although it has anti-inflammatory properties and supports immune homeostasis, its effect on collagen-induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3'-SL on the progression of rheumatoid arthritis (RA) in in vitro and in vivo models. The anti-arthritic effect of 3'-SL was analysed with fibroblast-like synoviocytes in vitro and an in vivo mouse model of CIA. RT-PCR, Western blotting and ELISA were performed to evaluate its effects in vitro. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin-O and haematoxylin staining. 3'-SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3'-SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro-inflammatory cytokines, matrix metalloproteinases and osteoclastogenesis via NF-κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF-κB signalling pa...Continue Reading

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Citations

May 31, 2019·Journal of Cellular and Molecular Medicine·Chanmi ChoSiyoung Yang
Sep 12, 2020·Critical Reviews in Food Science and Nutrition·Emmanuel Pérez-EscalanteLuis Guillermo González-Olivares
Jun 13, 2020·Journal of Cellular and Molecular Medicine·Hyemi LeeLi-Jung Kang
Dec 12, 2019·Neural Regeneration Research·Shuang-Xi ChenWei-Jiang Zhao
Feb 11, 2021·Pharmaceuticals·Hyemi LeeSiyoung Yang

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Methods Mentioned

BETA
dissection
flow cytometry
Assay
ELISA
light microscopy
PCR
FACS

Software Mentioned

ARRIVE
PRISM
ImageJ
MassHunter Data Acquisition
GenScript

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