31P and 13C solid-state NMR spectroscopy to study collagen synthesis and biomineralization in polymer-based bone implants

NMR in Biomedicine
Franziska WeberDaniel Huster

Abstract

A combination of solid-state NMR spectroscopy and MRI was used to evaluate the formation of extracellular matrix in poly(D,L-lactide-co-glycolide) (PLGA) bone implants. Porous PLGA scaffolds were implanted into rat tibiae and analysed after 2, 4 or 8 weeks. MRI clearly delineated the implants within the cancellous bone. Differences in the trabecular structure of the implanted material and native bone were demonstrated. In addition, implants were analyzed by solid-state NMR spectroscopy under magic angle spinning. (13)C NMR spectra showed the unambiguous signature of collagen formed in the scaffolds, but also the characteristic signals of the PLGA matrix, indicating that resorption was not complete after 8 weeks. Furthermore, (31)P NMR spectroscopy detected the inorganic component of the matrix, which is composed of bioapatite. (31)P NMR spectra were quantified and this analysis revealed that the amount of inorganic extracellular matrix formed de novo was significantly lower than in native bone. This demonstrates that solid-state NMR spectroscopy, in particular in combination with MRI, can provide useful information on the composition and structure of the extracellular matrix, and serve as a tool to evaluate the quality of tissu...Continue Reading

References

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J S Waugh
Nov 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·X MarchandiseA P Legrand
Mar 16, 1990·Biochemical and Biophysical Research Communications·R Fujisawa, Y Kuboki
Dec 1, 1988·Journal of Pharmaceutical Sciences·T S Wiedmann
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L S BatchelderD A Torchia
Apr 10, 1980·Nature·L W JelinskiD A Torchia
Aug 1, 1996·Clinical Orthopaedics and Related Research·E D ArringtonN A Davino
Oct 23, 1997·Journal of Biomedical Materials Research·M OkumuraS Tabata
Jul 17, 1998·Nature Structural Biology·R G Griffin
Aug 11, 1998·The Journal of Bone and Joint Surgery. American Volume·S P BruderS Kadiyala
Jan 1, 1991·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·N NishimuraS Yoshihara
Jul 8, 1999·Biomaterials·C E HolyM S Shoichet
Jan 27, 2000·The Journal of Trauma·T J BlokhuisH J Haarman
Jun 22, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·W GründerA Werner
Sep 6, 2000·Nature Biotechnology·H PetiteG Guillemin
Sep 16, 2000·Carbohydrate Research·L NajiK Arnold
Nov 9, 2000·Biomaterials·D W Hutmacher
Dec 29, 2000·The Journal of Bone and Joint Surgery. British Volume·M Hamadouche, L Sedel
Feb 24, 2001·The New England Journal of Medicine·R QuartoM Marcacci
Dec 18, 2001·Journal of Biomedical Materials Research·Noriyuki TamaiHideki Yoshikawa
Sep 28, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Daniel HusterKlaus Arnold
Jun 18, 2003·Journal of Magnetic Resonance·Corey R Morcombe, Kurt W Zilm
Jan 28, 2004·Magnetic Resonance in Chemistry : MRC·Detlef ReichertDaniel Huster
Jan 19, 2005·Cells, Tissues, Organs·Stefan RammeltHans Zwipp
May 12, 2005·The International Journal of Biochemistry & Cell Biology·Simon M Cool, Victor Nurcombe
Oct 22, 2005·Orthodontics & Craniofacial Research·M MastrogiacomoR Cancedda
Jan 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kristen R Taylor, Richard L Gallo
Jul 11, 2006·NMR in Biomedicine·Göran Zernia, Daniel Huster
Oct 17, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·S AnumulaF W Wehrli
Nov 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Himadri S GuptaPeter Fratzl
May 8, 2007·Biomaterials·Michael HackerMichaela B Schulz

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Citations

Sep 8, 2012·The Journal of Physical Chemistry. B·Kamal H MroueAyyalusamy Ramamoorthy
Jan 15, 2014·Progress in Nuclear Magnetic Resonance Spectroscopy·Christian BonhommeDanielle Laurencin
Apr 12, 2014·Tissue Engineering. Part B, Reviews·Manuela VenturaX Frank Walboomers
Nov 21, 2012·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Anja PenkDaniel Huster
Dec 23, 2014·The Journal of Physical Chemistry. B·Ratan Kumar RaiNeeraj Sinha
Oct 17, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Ondřej NikelDeepak Vashishth
Jul 5, 2016·Physical Chemistry Chemical Physics : PCCP·Kamal H MroueAyyalusamy Ramamoorthy
Jul 5, 2012·Journal of Tissue Engineering and Regenerative Medicine·Ronny M SchulzDaniel Huster
Sep 10, 2015·Magnetic Resonance in Chemistry : MRC·Chandan SinghNeeraj Sinha
Jan 24, 2017·Applied Magnetic Resonance·Joanna KrukJacek Kujawski

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