PMID: 10672034Feb 15, 2000Paper

31P NMR analysis of the DNA conformation induced by protein binding SRY/DNA complexes

European Journal of Biochemistry
C CastagnéM Delepierre


Complexes of the HMG box protein SRY with two duplexes of 8 and 14 base pairs have been studied by 31P NMR and complete assignment of all phosphorus signals of the bound DNA duplexes are presented. While for the free DNA, all 31P signals display limited spectral dispersion (< 0.8 p.p.m.) for the bound duplexes, 31P resonances are spread over 2 p.p.m. Based on the previously published 3D structure of hSRY-HMG, with the 8 mer it is demonstrated that the upfield shifted resonances correspond to the site of partial intercalation of an isoleucine side chain into the DNA. Moreover, the observation of significant difference in linewidths between the two duplexes allows to estimate lifetime of the complexes from 31P-31P 2D exchange experiments.


Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Guéron, R G Shulman
Mar 31, 1976·Journal of the American Chemical Society·D G GorensteinR K Momii
Sep 1, 1992·Molecular Microbiology·R E Harrington
Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Jul 1, 1992·Genomics·K D McElreaveyM Fellous
Aug 1, 1988·Journal of Biomolecular Structure & Dynamics·R Lavery, H Sklenar
Dec 15, 1986·European Journal of Biochemistry·M DelepierreB P Roques
Oct 11, 1995·Nucleic Acids Research·A Amir-AslaniS Fermandjian
Jan 1, 1993·Annual Review of Genetics·P N Goodfellow, R Lovell-Badge
Apr 25, 1994·Nucleic Acids Research·V R HarleyP N Goodfellow
Jan 1, 1994·Annual Review of Medicine·M L Gustafson, P K Donahoe
May 25, 1993·Nucleic Acids Research·V LaudetH Clevers
Jan 17, 1997·Journal of Molecular Biology·R A KumarD J Patel
Dec 15, 1971·FEBS Letters·M Guéron

❮ Previous
Next ❯


Dec 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joon-Hwa LeeFiona Jucker
Dec 25, 2003·Journal of Biomolecular Structure & Dynamics·Stéphane TeletcheaJirí Kozelka
Aug 11, 2006·Nucleosides, Nucleotides & Nucleic Acids·Cynthia M Dupureur
Dec 20, 2002·Nucleic Acids Research·Péter VárnaiBrigitte Hartmann
Mar 11, 2011·Journal of the Royal Society, Interface·S CampagneA Milon
Jul 30, 2009·Journal of the American Chemical Society·Young-Min KangJoon-Hwa Lee
Oct 9, 2009·Chemistry : a European Journal·Stéphane TéletchéaJirí Kozelka
Apr 21, 2009·The Journal of Physical Chemistry. B·Guillaume P H SantiniCatherine Hervé du Penhoat
Nov 8, 2001·Journal of the American Chemical Society·B Luy, J P Marino
Jul 13, 2006·Journal of the American Chemical Society·Brahim HeddiBrigitte Hartmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.