PMID: 3754772May 29, 1986Paper

31P-NMR spectra of methanogens: 2,3-cyclopyrophosphoglycerate is detectable only in methanobacteria strains

Biochimica Et Biophysica Acta
C J TolmanL Daniels

Abstract

The unique compound 2,3-cyclopyrophosphoglycerate occurs at a detectable concentration in the genera Methanobacterium and Methanobrevibacter but not in Methanococcus, Methanospirillum and Methanosarcina, as shown by a 31P-NMR survey of several different methanogens. Metabolic poisons (carbonyl cyanide m-chlorophenylhydrazone and valinomycin) do not decrease the level of the cyclic pyrophosphate in Methanobacterium thermoautotrophicum; therefore, it cannot be a phosphagen, i.e., an energy storage material. 13CO2 is rapidly incorporated into this cyclic compound which represents the major soluble carbon as well as the phosphorus component of this methanobacteria. 13C-NMR analysis demonstrates that the pKa of the 2,3-cyclopyrophosphoglycerate carboxyl group is 2.55. The unusual pseudomurein cell wall structure of methano- and methanobrevibacteria necessitates a high demand on carbohydrate metabolism. For this reason, and the fact that when its concentration is decreased no new phosphorus resonances appear in the high resolution spectra, it is suggested that 2,3-cyclopyrophosphoglycerate has a function in carbohydrate metabolism.

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Citations

Mar 15, 1989·European Journal of Biochemistry·H SantosA V Xavier
Jan 1, 1989·Critical Reviews in Microbiology·K F Jarrell, S F Koval
Jun 13, 2020·Analytical Chemistry·Fatema BhinderwalaRobert Powers
Jun 1, 1994·Journal of Bacteriology·R CiullaM F Roberts
Mar 1, 1997·Applied and Environmental Microbiology·L O MartinsH Santos

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