PMID: 6979940Jul 1, 1982Paper

31P nuclear magnetic resonance analysis of frog skin

The American Journal of Physiology
L E LinM M Civan

Abstract

The intracellular phosphate composition of whole and split frog skins has been studied by 31P nuclear magnetic resonance (NMR) analysis. The spectra were similar to those previously recorded from isolated epithelial cells of toad bladder. However, qualitative differences were noted in comparison with spectra from whole toad bladder. The 31P spectra from whole frog skin reflect the intracellular compositions of the epithelial cells, whereas subepithelial elements contribute significantly to the total observed 31P signals from toad bladder. Analyzed at 4 degrees C, the average phosphocreatine (PCr) and ATP concentrations of frog skin are of similar magnitude. The ratio of [PCr] to [ATP + ADP] depends on time, tissue oxygen tension, temperature, and extracellular inorganic phosphate concentration. Both this ratio and the short-circuit current (measured in parallel experiments) fell during the course of aerating frog skins in Ringer solution at room temperature. The intracellular inorganic phosphate (Pi) signal was identified. After reduction of extracellular pH, the signal did not shift immediately but subsequently did undergo an acid shift.

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Citations

Jun 1, 1988·Journal of Electron Microscopy Technique·K M MadsenC C Tisher
Jan 1, 1985·Archives of Dermatological Research·P Verrando, J P Ortonne
Aug 1, 1996·NMR in Biomedicine·A G CowieG K Radda
Apr 1, 1986·Diabetologia·G AugertY Le Marchand-Brustel
Jul 1, 1990·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·L P BrionG J Schwartz
Aug 1, 1990·Pflügers Archiv : European journal of physiology·Y MatsushimaM Imai
Dec 18, 2010·Obesity·Christos S Katsanos, Lawrence J Mandarino
Mar 1, 1984·The American Journal of Physiology·M ShporerM M Civan
Nov 1, 1984·The American Journal of Physiology·M M CivanC Deutsch
Sep 1, 1983·The American Journal of Physiology·M M CivanM Shporer
Apr 1, 1987·Biochimie·T GremeauxY Le Marchand-Brustel
Aug 1, 1984·Molecular and Cellular Endocrinology·S Monier, Y Le Marchand-Brustel
Nov 15, 1988·Analytical Biochemistry·L P BrionG J Schwartz

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