31P nuclear magnetic resonance analysis of phospholipids in a ternary homogeneous system

Analytical Biochemistry
M BrancaM V Serra

Abstract

We analyzed the changes in the shape and resonance positions of the 31P NMR signals of phospholipid mixtures in a single-phase solvent containing different quantities of chloroform, methanol, and water. We obtained extremely reproducible NMR signals when sufficient care was taken to control the desired solvent composition. The solvent composition can be predicted using a "tie line" in a ternary diagram of chloroform, methanol, and water or by mixing the three components at the desired ratio. Avoiding the presence of a biphasic system allows easy solvent preparation and NMR spectral reproducibility, with the possibility of complete recovery of the sample and absence of loses due to different repartition coefficients.

Citations

Feb 24, 2010·Biotechnology and Bioengineering·C M BealR E Hebner
Nov 21, 2009·Biochimica Et Biophysica Acta·Rosendo EstradaM Cecilia Yappert
Aug 5, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J M Pearce, R A Komoroski
Dec 1, 2013·Biophysics Reviews·Norbert W Lutz, Patrick J Cozzone
Oct 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Dongyu ZhangAnn E McDermott
Jan 19, 2019·Analytical Chemistry·Boris GouilleuxThomas Vosegaard

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