PMID: 6990986Apr 24, 1980Paper

31P nuclear magnetic resonance and freeze-fracture electron microscopy studies on Escherichia coli. II. Lipopolysaccharide and lipopolysaccharide-phospholipid complexes

Biochimica Et Biophysica Acta
L van AlphenB Lugtenberg

Abstract

1. Freeze-fracture electron microscopy and 31P-NMR spectroscopy on native and electrodialyzed lipopolysaccharide from Escherichia coli K12 cells, both above and below the phase transition temperature, are described. 2. Freeze-fracture electron microscopy of native lipopolysaccharide shows ribbon-like structures below (0 and 22 degrees C) and large vesicles above (37 degrees C) the phase transition temperature. Electrodialyzed lipopolysaccharide (sodium salt) occurs in ribbon-like structures at 0, 22 and 37 degrees C if sodium lipopolysaccharide is hydrated in water. If sodium lipopolysaccharide is hydrated in Tris-HCL/NaCl buffer these ribbon-like structures occur only below the phase transition temperature. Above the phase transition temperature stacked sheets are observed. Moreover, in the latter case, the fracture planes contain particles and pits. Upon etching, sodium lipopolysaccharide when hydrated in water appears to form rods and when hydrated in buffer appears to form mainly stacked lamellae both above (37 degrees C) and below (0 degrees C) the phase transition temperature. 3. High resolution 31P-NMR spectra show that the chemical shifts of the phosphorus atoms in native lipopolysaccharide differ from those in electrod...Continue Reading

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Citations

Feb 1, 1984·The Journal of Experimental Medicine·M S Blake, E C Gotschlich
Dec 4, 2004·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yasuyuki Asai
Mar 15, 1985·Biochemical and Biophysical Research Communications·A G SabelnikovI A Vasilenko
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May 1, 1981·Journal of Bacteriology·H E BergmansW P Hoekstra
May 3, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Amanda J Brindley, Rachel W Martin

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