PMID: 6972869May 1, 1981Paper

31P nuclear magnetic resonance studies of energy transduction in Rhodopseudomonas sphaeroides

European Journal of Biochemistry
K NicolayW N Konings

Abstract

31P nuclear magnetic resonance spectra of th phototrophic bacterium Rhodopseudomonas sphaeroides reveal the presence of inorganic phosphate, sugar phosphates and two non-identified P,P1-diesterified pyrophosphate compounds. Due to the presence of paramagnetic cations the resonances of these compounds can only be detected after repeated washing of the bacterial cells with a buffer, containing EDTA plus excess Mg2+. Washing with Mg2+-free EDTA buffer deteriorates the structural integrity of the membranes of Rps. sphaeroides. This is indicated by the appearance of an extra resonance peak in the spectra of these cells in a region where the phospholipids absorb and by a fivefold increase in proton permeability of the cytoplasmic membrane of Rps. sphaeroides under these conditions. Upon illumination of the cell suspension in the NMR tube the generation of a transmembrane pH gradient can be inferred from the shift in the resonances of extracellular and intracellular inorganic phosphate. Intracellular inorganic phosphate shows one homogeneous resonance peak upon illumination. This demonstrates that the mixing system, which has been developed for this application, functions efficiently. The magnitude of the light-dependent pH difference...Continue Reading

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Citations

Jan 1, 1986·Photosynthesis Research·D B Knaff
Sep 1, 1982·Molecular and Biochemical Parasitology·A G TielensS G van den Bergh
Jun 19, 2002·Journal of Microbiological Methods·Luísa S SerafimMaria A M Reis
Jan 1, 1984·The American Journal of Physiology·R S Balaban
Jun 1, 1982·Journal of Bacteriology·K J HellingwerfW N Konings
Dec 1, 1988·Journal of Bacteriology·P S Poole, J P Armitage
Nov 15, 1984·Archives of Biochemistry and Biophysics·B A MelandriL Packer

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