3,2'-Dihydroxyflavone Improves the Proliferation and Survival of Human Pluripotent Stem Cells and Their Differentiation into Hematopoietic Progenitor Cells

Journal of Clinical Medicine
Kyeongseok KimSsang-Goo Cho

Abstract

Efficient maintenance of the undifferentiated status of human pluripotent stem cells (hiPSCs) is crucial for producing cells with improved proliferation, survival and differentiation, which can be successfully used for stem cell research and therapy. Here, we generated iPSCs from healthy donor peripheral blood mononuclear cells (PBMCs) and analyzed the proliferation and differentiation capacities of the generated iPSCs using single cell NGS-based 24-chromosome aneuploidy screening and RNA sequencing. In addition, we screened various natural compounds for molecules that could enhance the proliferation and differentiation potential of hiPSCs. Among the tested compounds, 3,2'-dihydroxyflavone (3,2'-DHF) significantly increased cell proliferation and expression of naïve stemness markers and decreased the dissociation-induced apoptosis of hiPSCs. Of note, 3,2'-DHF-treated hiPSCs showed upregulation of intracellular glutathione (GSH) and an increase in the percentage of GSH-high cells in an analysis with a FreSHtracer system. Interestingly, culture of the 3,2'-DHF-treated hiPSCs in differentiation media enhanced their mesodermal differentiation and differentiation into CD34+ CD45+ hematopoietic progenitor cells (HPC) and natural kill...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·N S Kosower, E M Kosower
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 29, 2003·The Journal of Nutritional Biochemistry·Kelly E. HeimDennis J. Bobilya
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yong ZhaoEliezer Huberman
Oct 30, 2004·Yonsei Medical Journal·Gulizar Atmaca
Mar 18, 2005·Annual Review of Immunology·Lewis L Lanier
Jul 15, 2005·The Journal of Biological Chemistry·Eung-Ryoung LeeSsang-Goo Cho
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Apr 6, 2006·Cancer Research·Fazlul H Sarkar, Yiwei Li
Aug 15, 2006·Biochimica Et Biophysica Acta·Eung-Ryoung LeeSsang-Goo Cho
Jan 5, 2007·Biological & Pharmaceutical Bulletin·Eung-Ryoung LeeSsang-Goo Cho
Apr 18, 2007·Nature Reviews. Immunology·Hans-Gustaf Ljunggren, Karl-Johan Malmberg
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jun 6, 2009·Cell Stem Cell·Jennifer Nichols, Austin Smith
Oct 20, 2009·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Noemi FusakiMamoru Hasegawa
Dec 9, 2009·The Biochemical Journal·Jungwoon LeeJungho Kim
Jul 21, 2010·Nature·K KimG Q Daley
Nov 13, 2010·The Journal of Reproduction and Development·Keum Sil LeeSe Pill Park
Jan 8, 2011·Science·Eric VivierSophie Ugolini
Mar 19, 2011·Cell·Richard A Young
Jun 22, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Eung-Ryoung LeeSsang-Goo Cho
Dec 20, 2011·Experimental & Molecular Medicine·Jiho JangDong-Wook Kim
Jan 17, 2012·Muscle & Nerve·Carlos B Mantilla, Leonid G Ermilov
Jun 19, 2012·Cell Stem Cell·Shinya Yamanaka
Dec 12, 2012·Nature Biotechnology·Cole TrapnellLior Pachter
Mar 16, 2013·Cell Death & Disease·Kui WangYuquan Wei
Nov 26, 2013·Biochimica Et Biophysica Acta·Sonia CortassaMiguel A Aon
Dec 18, 2013·Cellular & Molecular Immunology·Zhengping JiangXuetao Cao
Jan 29, 2014·TheScientificWorldJournal·Shashank Kumar, Abhay K Pandey
May 30, 2014·The Journal of Microbiology·Mohammed Kawser HossainSsang-Goo Cho
Aug 16, 2014·Cell·Patrick CahanJames J Collins
Sep 26, 2014·Stem Cell Reports·Mi-Yoon ChangSang-Hun Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
PCR
RNA-seq
Flow Cytometry
Assay
fluorescence-activated cell sorting
FACS
protein
electrophoresis
Fluorescence

Software Mentioned

GraphPad Prism
BlueFuse Multi for
Cuffdiff
MiSeq Reporter
g Profiler
Cufflinks
house
R scripts

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.