32P-post-labelling analysis of DNA adducts formed by aristolochic acid in tissues from patients with Chinese herbs nephropathy

Carcinogenesis
C A BielerHeinz H Schmeiser

Abstract

Recently, we reported that aristolochic acid (AA) a naturally occurring nephrotoxin and carcinogen is implicated in a unique type of renal fibrosis, designated Chinese herbs nephropathy (CHN). Indeed, we identified the principal aristolochic acid-DNA adduct in the kidney of five such patients. We now extend these observations and demonstrate the presence of additional AA-DNA adducts by the 32P-post-labelling method not only in the kidneys, but also in a ureter obtained after renal transplantation. Using the nuclease P1 version of the assay not only the major DNA adduct of aristolochic acid, 7-(deoxyadenosin-N6-yl)-aristolactam I (dA-AAI), but also the minor adducts, 7-(deoxyguanosin-N2-yl)-aristolactam I (dG-AAI) and 7-(deoxyadenosin-N6-yl)-aristolactam II (dA-AAII) were detected, and identified by cochromatographic analyses with TLC and HPLC. Quantitative analyses of six kidneys revealed relative adduct levels from 0.7 to 5.3/10(7) for dA-AAI, from 0.02 to 0.12/10(7) for dG-AAI and 0.06 to 0.24/ 10(7) nucleotides for dA-AAII. The detection of the dA-AAII adduct is consistent with the occurrence of aristolochic acid II (AAII) in the herb powder imported under the name of Stephania tetrandra and confirms that the patients had in...Continue Reading

Citations

Sep 7, 2002·International Journal of Cancer. Journal International Du Cancer·Volker M ArltJean-Pierre Cosyns
Jul 4, 2001·International Journal of Cancer. Journal International Du Cancer·V M ArltH H Schmeiser
Dec 15, 2010·Analytical and Bioanalytical Chemistry·Han-Zhi WuZongwei Cai
Jan 9, 2007·Journal of the American Society for Mass Spectrometry·Wan ChanZongwei Cai
Mar 23, 2002·Mutation Research·Arihiro KoharaMakoto Hayashi
Aug 3, 2004·Nature Reviews. Cancer·Miriam C Poirier
May 31, 2001·Kidney International·J P CosynsC van Ypersele de Strihou
Oct 9, 2003·Kidney International·Gaëlle GillerotOlivier Devuyst
Jun 8, 2000·The New England Journal of Medicine·J L NortierJ L Vanherweghem
Jun 17, 1998·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·A Singer, B Reid
Jun 26, 1998·Journal of Clinical Laser Medicine & Surgery
Mar 3, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Katerina LevováMarie Stiborová
Jul 9, 2010·Nephrology·Vivekanand Jha
Jan 12, 2010·Arhiv za higijenu rada i toksikologiju·Annie Pfohl-Leszkowicz
Jan 18, 2013·Advanced Biomedical Research·Mohammad Asif
Sep 17, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Marie StiborovaVilim Simanek
Apr 3, 2014·Natural Product Reports·Johanna MichlMichael Heinrich
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhipei LiuMonica Hollstein
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Arthur P GrollmanBojan Jelaković
Dec 24, 2002·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Jean-Pierre Cosyns
Mar 8, 2013·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Jian ZhangUNKNOWN China National Survey of Chronic Kidney Disease Working Group
Aug 10, 2004·International Journal of Cancer. Journal International Du Cancer·Volker M ArltJean-Pierre Cosyns
Jan 21, 2014·Chemical Research in Toxicology·Silvia BalboPeter W Villalta
Dec 24, 2005·Critical Care Medicine·Greg S MartinMarc Moss
Mar 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wan ChanZongwei Cai

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