PMID: 9174270Apr 11, 1997Paper

32P-Postlabeling high-performance liquid chromatographic improvements to characterize DNA adduct stereoisomers from benzo[a]pyrene and benzo[c]phenanthrene, and to separate DNA adducts from 7,12-dimethylbenz[a]anthracene

Journal of Chromatography. B, Biomedical Sciences and Applications
M Zeisig, L Möller

Abstract

Single compounds can generate complex DNA adduct patterns by reactions through different pathways, with different target nucleotides and through different configurations of the products. DNA adduct analysis by 32P-HPLC was improved by adding an isocratic plateau in an otherwise linear gradient, thereby enhancing resolution of predictable retention time intervals. This enhanced 32P-HPLC technique was used to analyze and at least partly resolve 14 out of 16 available benzo[c]phenanthrene deoxyadenosine and deoxyguanosine adduct standards, 8 out of 8 available benzo[a]pyrene deoxyadenosine and deoxyguanosine adduct standards, and 51 peaks from 7,12-dimethylbenz[a]anthracene-calf thymus DNA reaction products. The same type of gradient modifications could be used to enhance resolution in analyses of other complex DNA adduct mixtures, e.g., in vivo in humans.

References

Jul 1, 1992·Carcinogenesis·A C Beach, R C Gupta
Mar 1, 1990·Chemical Research in Toxicology·J F MouretJ Cadet

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Citations

Jun 25, 2002·Molecular Aspects of Medicine·Helen R GriffithsSiân B Astley
Dec 23, 2008·Mutagenesis·Eszter NagyLennart Möller
Jun 13, 2002·Environmental Health Perspectives·Carl-Elis BoströmRoger Westerholm
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Gunther GuetensErnst A de Bruijn
May 19, 2000·The Proceedings of the Nutrition Society·H E PoulsenS Loft

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