3,4-Dihydroxy-L-Phenylalanine as a Novel Covalent Linker of Extracellular Matrix Proteins to Polyacrylamide Hydrogels with a Tunable Stiffness

Tissue Engineering. Part C, Methods
Olaf Y WoutersRuud A Bank

Abstract

Cells acquire mechanical information from their surrounding and convert this into biochemical activity. The concept and mechanism behind this cellular mechanosensing and mechanotransduction are often studied by means of two-dimensional hydrogels. Polyacrylamide hydrogels (PAAMs) offer chemical, mechanical, and optical advantages but due to their inert surface do not allow protein and cell adherence. Several cross-linkers have been used to functionalize the surface of PAAMs with extracellular matrix (ECM) proteins to enable cell culture. However, the most commonly used cross-linkers are either unstable, expensive, or laborious and often show heterogeneous coating or require PAAM modification. Here, we introduce 3,4-dihydroxy-l-phenylalanine (L-DOPA) as a novel cross-linker that can functionalize PAAMs with ECM without the above-mentioned disadvantages. A homogenous collagen type I and fibronectin coating was observed after L-DOPA functionalization. Fibroblasts responded to differences in PAAMs' stiffness; morphology, cell area, and protein localization were all affected as expected, in accordance with literature where other cross-linkers were used. In conclusion, L-DOPA can be used as a cross-linker between PAAMs and ECM and rep...Continue Reading

References

Oct 31, 1980·Biochemical and Biophysical Research Communications·J H Waite, M L Tanzer
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Feb 25, 1997·Experimental Cell Research·R M EzzellD E Ingber
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J Pelham, Y l Wang
Apr 10, 1999·Molecular Biology of the Cell·R J Pelham, Y l Wang
Apr 21, 1999·Current Opinion in Cell Biology·S M Schoenwaelder, K Burridge
Sep 9, 1999·The Journal of Cell Biology·I KaverinaJ V Small
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Sep 22, 2000·Biochemistry·L A Burzio, J H Waite
Oct 13, 2000·American Journal of Physiology. Cell Physiology·H B WangY L Wang
Aug 2, 2002·Cell·Benjamin Geiger, Alexander Bershadsky
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexander D BershadskyBenjamin Geiger
Jul 17, 2004·Annual Review of Biomedical Engineering·Christopher S ChenJoe Tien
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
May 13, 2005·Nature·Cornelis StormPaul A Janmey
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Dec 20, 2005·European Journal of Cell Biology·Alexander D BershadskyMichael M Kozlov
Jan 13, 2006·The Journal of Cell Biology·Jérôme M GoffinBoris Hinz
May 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Donald E Ingber
Aug 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Haeshin LeePhillip B Messersmith
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Jul 7, 2007·Methods in Cell Biology·Casey E KandowKaren A Beningo
Sep 29, 2007·Advanced Drug Delivery Reviews·Florian RehfeldtDennis E Discher
Oct 20, 2007·Science·Haeshin LeePhillip B Messersmith
Nov 30, 2007·Biophysical Journal·Jérôme SolonPaul A Janmey
May 21, 2010·Nature·Haeshin Lee
Dec 16, 2010·Biophysical Journal·Krishanu SahaRyan C Hayward
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Sunyoung LeeIra M Herman
Jan 1, 2009·The Journal of Adhesion·Murat GuvendirenKenneth R Shull
Jun 10, 2011·Nature·Sirio DupontStefano Piccolo
May 15, 2012·Biotechnology Progress·Wan-Geun LaByung-Soo Kim
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Feb 23, 2013·Current Biology : CB·Emma Spanjaard, Johan de Rooij
Apr 11, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Yiran LiWei Wang
Feb 21, 2008·Soft Matter·Verónica TrujilloRyan C Hayward

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Citations

May 5, 2018·Cell and Tissue Research·Bram PiersmaRuud A Bank
May 22, 2021·Cell Metabolism·Kevin M TharpValerie M Weaver
Oct 22, 2021·Journal of Inherited Metabolic Disease·Vivian LehmannSabine A Fuchs

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Methods Mentioned

BETA
fluorescence microscopy
AFM
Infrared Imaging
atomic force microscopy
Fluorescence

Software Mentioned

GraphPad
GraphPad Prism
Leica NuanceFX
FIJI
ImageJ
TissueGnostics TissueFAXS
LI
COR image studio

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