3,5,6,7,8,3',4'-Heptamethoxyflavone, a Citrus Flavonoid, Inhibits Collagenase Activity and Induces Type I Procollagen Synthesis in HDFn Cells

International Journal of Molecular Sciences
Hong-Il KimYoung-Jin Park

Abstract

Citrus fruits contain various types of flavonoids with powerful anti-aging and photoprotective effects on the skin, and have thus been attracting attention as potential, efficacious skincare agents. Here, we aimed to investigate the chemical composition of Citrus unshiu and its protective effects on photoaging. We isolated and identified a bioactive compound, 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF), from C. unshiu peels using ethanol extraction and hexane fractionation. HMF inhibited collagenase activity and increased type I procollagen content in UV-induced human dermal fibroblast neonatal (HDFn) cells. HMF also suppressed the expression of matrix metalloproteinases 1 (MMP-1) and induced the expression of type I procollagen protein in UV-induced HDFn cells. Additionally, HMF inhibited ultraviolet B (UVB)-induced phosphorylation of the mitogen-activated protein kinases (MAPK) cascade signaling components-ERK, JNK, and c-Jun-which are involved in the induction of MMP-1 expression. Furthermore, HMF affected the TGF-β/Smad signaling pathway, which is involved in the regulation of type I procollagen expression. In particular, HMF induced Smad3 protein expression and suppressed Smad7 protein expression in UV-induced HDFn cells in ...Continue Reading

References

Jan 1, 1990·Annals of the New York Academy of Sciences·A B RobertsM B Sporn
Mar 1, 1988·Biochemical Pharmacology·J Robak, R J Gryglewski
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R P PenttinenP Bornstein
Jul 1, 1993·Journal of Periodontology·M MakimuraS Otake
Jan 15, 1993·American Journal of Medical Genetics·J L SlackP Bornstein
Jan 1, 1996·Chemical Research in Toxicology·R L Divi, D R Doerge
Jan 1, 1997·Free Radical Biology & Medicine·G CaoR L Prior
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·W D ShingletonT E Cawston
May 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S C Sahu, G C Gray
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·J N TopperD Falb
Dec 12, 1997·Biochimica Et Biophysica Acta·A Minden, M Karin
Sep 18, 1998·Biochemistry and Molecular Biology International·E DickancaitéN Cènas
Feb 11, 1999·Journal of the American Dietetic Association·A King, G Young
Apr 1, 2000·Clinical and Experimental Pharmacology & Physiology·L W MortonK D Croft
Oct 18, 2000·Free Radical Biology & Medicine·C F Skibola, M T Smith
Feb 24, 2001·Drug Metabolism and Drug Interactions·C Rice-EvansA R Rechner
Mar 1, 2002·Mechanisms of Ageing and Development·Gail Jenkins
Sep 5, 2002·Ageing Research Reviews·Laure Rittié, Gary J Fisher
May 2, 2003·Journal of Agricultural and Food Chemistry·Rebecca J Robbins
Nov 5, 2003·Gene·Shen-Hsi YangAlan J Whitmarsh
Nov 19, 2003·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Mar 24, 2004·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Alena SvobodováDaniela Walterová
Feb 3, 2005·Biological & Pharmaceutical Bulletin·Mitsuyoshi MatsuoTakao Kaneko
Aug 3, 2006·Experimental Dermatology·Farrukh Afaq, Hasan Mukhtar
Feb 16, 2016·Toxicological Research·Bi Gyeon HaYoung Chul Kim
Mar 31, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Agata Jabłońska-TrypućStanisław Rosochacki
Nov 2, 2016·International Journal of Molecular Sciences·Ji-Woong KimYoung-Jin Park
Jul 29, 2017·Archives of Oral Biology·Zhengya LiuJihua Chen

❮ Previous
Next ❯

Citations

Mar 28, 2020·Nutrients·Changwei CaoChangrong Ge
Apr 23, 2020·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Asmaa Aboul NaserManal Hamed
Aug 30, 2020·International Journal of Molecular Sciences·Daniel A Jacobo-Velázquez, Luis Cisneros-Zevallos

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
column chromatography
NMR
electrophoresis

Software Mentioned

TL
Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.