3D-3-culture: A tool to unveil macrophage plasticity in the tumour microenvironment

Biomaterials
Sofia P RebeloCatarina Brito

Abstract

The tumour microenvironment (TME) shapes disease progression and influences therapeutic response. Most aggressive solid tumours have high levels of myeloid cell infiltration, namely tumour associated macrophages (TAM). Recapitulation of the interaction between the different cellular players of the TME, along with the extracellular matrix (ECM), is critical for understanding the mechanisms underlying disease progression. This particularly holds true for prediction of therapeutic response(s) to standard therapies and interrogation of efficacy of TME-targeting agents. In this work, we explored a culture platform based on alginate microencapsulation and stirred culture systems to develop the 3D-3-culture, which entails the co-culture of tumour cell spheroids of non-small cell lung carcinoma (NSCLC), cancer associated fibroblasts (CAF) and monocytes. We demonstrate that the 3D-3-culture recreates an invasive and immunosuppressive TME, with accumulation of cytokines/chemokines (IL4, IL10, IL13, CCL22, CCL24, CXCL1), ECM elements (collagen type I, IV and fibronectin) and matrix metalloproteinases (MMP1/9), supporting cell migration and promoting cell-cell interactions within the alginate microcapsules. Importantly, we show that both t...Continue Reading

Citations

May 31, 2018·Human Vaccines & Immunotherapeutics·Yiming MengHaozhe Piao
Jul 3, 2020·Alternatives to Laboratory Animals : ATLA·Elisabete Nascimento-GonçalvesBruno Jorge Antunes Colaço
Mar 23, 2019·Journal of Experimental & Clinical Cancer Research : CR·Francesca Di ModugnoPaola Nisticò
Aug 19, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ana Luísa CartaxoCatarina Brito
Apr 4, 2019·Metallomics : Integrated Biometal Science·Debora WernitznigBernhard K Keppler
Apr 10, 2020·Frontiers in Oncology·Christophe BlanquartDidier Jean
Mar 19, 2020·Cells·Robin DemuynckDmitri V Krysko
May 3, 2020·Journal of Experimental & Clinical Cancer Research : CR·Somshuvra BhattacharyaPilar de la Puente
Jun 28, 2020·Journal of Leukocyte Biology·Rosario Luque-MartinMenno P J Winther
Oct 18, 2019·Journal of Clinical Medicine·Ilya YakavetsLina Bezdetnaya
Jun 9, 2020·Frontiers in Oncology·Erica J Hoffmann, Suzanne M Ponik
Nov 24, 2020·Frontiers in Bioengineering and Biotechnology·Bernardo AbecasisPaula M Alves
Dec 10, 2020·Metallomics : Integrated Biometal Science·Debora WernitznigBernhard K Keppler
Jan 14, 2021·Biomaterials Science·Kanishka FernandoEliza Li Shan Fong
Dec 11, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yana ShafranNaomi Zurgil
Feb 13, 2021·Advanced Healthcare Materials·Hyun Jeong OhSeok Chung
Nov 15, 2020·Nanomaterials·Indra Van ZundertSusana Rocha
Nov 20, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Tuli Dey
Nov 22, 2020·Trends in Cancer·João RodriguesJai Prakash
Nov 19, 2020·Scientific Reports·Shankar J EvaniKai P Leung
Feb 26, 2021·Frontiers in Immunology·Russell FoxallMark S Cragg
Mar 7, 2021·International Journal of Molecular Sciences·Nuno LopesCatarina Brito
May 1, 2021·Cancers·Elodie PramilAlexandre E Escargueil
Jun 3, 2021·Cancers·Magdalena RauschPatrycja Nowak-Sliwinska
Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Teresa Franchi-MendesCatarina Brito
Jun 26, 2021·Journal of Biomedical Nanotechnology·Wei JiangShuyan Zhao
Jul 2, 2021·Advanced Drug Delivery Reviews·Matthew L TanClaudia Fischbach
Aug 29, 2021·Pathogens·Natasha Helleberg MadsenJesper Larsen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Related Papers

Nihon rinsho. Japanese journal of clinical medicine
Yoshihiro Kawasaki, Tetsu Akiyama
Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
P MenèG Gambaro
© 2022 Meta ULC. All rights reserved