3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells

Journal of Neural Engineering
Ross A PetrellaPatrick C Sachs

Abstract

Picosecond pulse electric fields (psPEF) have the potential to elicit functional changes in mammalian cells in a non-contact manner. Such electro-manipulation of pluripotent and multipotent cells could be a tool in both neural interface and tissue engineering. Here, we describe the potential of psPEF in directing neural stem cells (NSCs) gene expression, metabolism, and proliferation. As a comparison mesenchymal stem cells (MSCs) were also tested. A psPEF electrode was anchored on a customized commercially available 3D printer, which allowed us to deliver pulses with high spatial precision and systematically control the electrode position in three-axes. When the electrodes are continuously energized and their position is shifted by the 3D printer, large numbers of cells on a surface can be exposed to a uniform psPEF. With two electric field strengths (20 and 40 kV cm-1), cell responses, including cell viability, proliferation, and gene expression assays, were quantified and analyzed. Analysis revealed both NSCs and MSCs showed no significant cell death after treatments. Both cell types exhibited an increased metabolic reduction; however, the response rate for MSCs was sensitive to the change of electric field strength, but for ...Continue Reading

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Citations

Jun 20, 2019·Journal of the Royal Society, Interface·Ram Anand VadlamaniAllen L Garner
Sep 17, 2019·Oncotarget·Robert D BrunoPatrick C Sachs
Jul 10, 2019·Experimental and Therapeutic Medicine·Ka WangShiyuan Xu
May 11, 2021·Stem Cells International·Hong ChengYubo Fan

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