3D bioprinting and in vitro study of bilayered membranous construct with human cells-laden alginate/gelatin composite hydrogels

Colloids and Surfaces. B, Biointerfaces
Pengchao LiuSteve Guofang Shen

Abstract

Extrusion-based 3D bioprinting of cell-laden hydrogels is a potential technology for regenerative medicine, which enables the fabrication of constructs with spatially defined cell distribution. However, the limited assessment of rheological behaviors of hydrogel before printing is still a major issue for the advancement of 3D bioprinting. In this work, we systematically investigated the rheological behaviors (i.e. viscosity, storage modulus (G'), and loss modulus (G")) of alginate/gelatin composite hydrogels first for 3D printing complex constructs. The rheological studies revealed that viscosity of alginate/gelatin hydrogels is temperature-dependent and shear thinning. Sol-gel transition (intersection of G' and G") study provided indication for printing temperature, which are in the range of 18.8 °C (H2/7.5) to 24.5 °C (H2/24.5). The alginate (2 wt%) /gelatin (15 wt%) composite hydrogel sample was chosen to print the constructs and subsequent bioprinting. Complex constructs (i.e. nose and ear) were obtained with high printing resolution (151 ± 13.04 μm) in a low temperature (4 °C) chamber and crosslinking with 2 wt% CaCl2 subsequently without extra supports. Human amniotic epithelial cells (AECs) showed superior potential to d...Continue Reading

Citations

Dec 21, 2019·Tissue Engineering. Part C, Methods·Tiago Ramos, Lorenzo Moroni
Sep 17, 2020·International Journal of Molecular Sciences·Arantza Perez-ValleIsabel Andia
Mar 2, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Filis CurtiIzabela-Cristina Stancu
Apr 23, 2021·Small·Sadjad KhosravimelalMazaher Gholipourmalekabadi
Jun 15, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Baosen TanXiaoming Li
Aug 24, 2021·Colloids and Surfaces. B, Biointerfaces·Chuang GaoYuanyuan Liu

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