3D bioprinting of mature bacterial biofilms for antimicrobial resistance drug testing

Biofabrication
Evita NingWenmiao Shu

Abstract

The potential to bioprint and study 3D bacterial biofilm constructs could have great clinical significance at a time when antimicrobial resistance is rising to dangerously high levels worldwide. In this study, clinically relevant bacterial species including Escherichia coli, Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa were 3D bioprinted using a double-crosslinked alginate bioink to form mature bacteria biofilms, characterized by confocal laser scanning microscopy (CLSM) and fluorescent staining. Solid and porous bacteria-laden constructs were reproducibly bioprinted with thicknesses ranging from 0.25 to 4 mm. We demonstrated 3D bioprinting of thicker biofilms (>4 mm) than found in currently available in vitro models. Bacterial viability was excellent in the bioprinted constructs, with CLSM observation of bacterial biofilm production and maturation possible for at least 28 d in culture. Importantly, we observed the complete five-step biofilm life cycle in vitro following 3D bioprinting for the first time, suggesting the formation of mature 3D bioprinted biofilms. Bacterial growth was faster in thinner, more porous constructs whilst constructs crosslinked with BaCl2 ...Continue Reading

References

May 21, 1999·Science·J W CostertonE P Greenberg
Aug 22, 2001·Journal of Bacteriology·M HentzerM R Parsek
Sep 21, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R M Donlan
Dec 4, 2002·American Journal of Infection Control·UNKNOWN National Nosocomial Infections Surveillance System
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sara E CosgroveYehuda Carmeli
May 9, 2006·Biomacromolecules·Yrr A MørchGudmund Skjåk-Braek
Sep 27, 2006·Environmental Science & Technology·Bruce E LoganKorneel Rabaey
Mar 28, 2008·Biotechnology and Bioengineering·James D Bryers
May 30, 2008·Clinical Reviews in Allergy & Immunology·Victoria E Wagner, Barbara H Iglewski
Dec 4, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sünje Johanna PampTim Tolker-Nielsen
Sep 5, 2009·Advances in Applied Microbiology·Andrew J McBain
Oct 28, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·James H Jorgensen, Mary Jane Ferraro
Feb 13, 2010·International Journal of Antimicrobial Agents·Niels HøibyOana Ciofu
Mar 11, 2010·Journal of Wound Care·R D WolcottS E Dowd
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis
Dec 18, 2010·Future Microbiology·Rasmus V RasmussenNiels E Bruun
Jun 15, 2011·Antimicrobial Agents and Chemotherapy·Wang HengzhuangNiels Høiby
Jan 12, 2013·Current Protein & Peptide Science·James A Garnett, Steve Matthews
Jan 30, 2013·Current Opinion in Microbiology·Kerry E BoyleJoao B Xavier
May 3, 2013·APMIS. Supplementum·Thomas Bjarnsholt
Jul 6, 2013·Trends in Microbiology·Thomas BjarnsholtNiels Høiby
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jodi L ConnellJason B Shear
Dec 5, 2013·BMC Infectious Diseases·Deborah A WilliamsonStephen R Ritchie
Dec 18, 2013·Acta Biomaterialia·Sílvia J BidarraPedro L Granja
Apr 29, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M D MaciàA Oliver
Dec 17, 2014·International Journal of Oral Science·Hong WuZhi-Jun Song
Jan 30, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I Olsen
Sep 8, 2015·Journal of Molecular Biology·Aled E L RobertsStephen P Diggle
Dec 23, 2015·Biofabrication·Atabak Ghanizadeh TabrizWenmiao Shu
Jan 9, 2016·Biofabrication·Jürgen GrollJos Malda
Mar 17, 2016·Journal of Microbiological Methods·Sebastian Schlafer, Rikke L Meyer
Mar 30, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Jean-Marc RolainDidier Raoult
Nov 22, 2016·Critical Reviews in Microbiology·Joana AzeredoClaus Sternberg
Feb 23, 2017·ACS Synthetic Biology·Benjamin A E LehnerAnne S Meyer
Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Werner Zimmerli, Parham Sendi

❮ Previous
Next ❯

Citations

Jul 10, 2020·Chemical Society Reviews·Maryam RahmatiHåvard J Haugen
Dec 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Adam Chyzy, Marta E Plonska-Brzezinska
Mar 8, 2021·Biosensors & Bioelectronics·Yanlin DengBee Luan Khoo
Jun 16, 2021·Biomaterials Science·Emily Diep, Jessica D Schiffman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.