3D bioprinting of neural stem cell-laden thermoresponsive biodegradable polyurethane hydrogel and potential in central nervous system repair

Biomaterials
Fu-Yu HsiehShan-Hui Hsu

Abstract

The 3D bioprinting technology serves as a powerful tool for building tissue in the field of tissue engineering. Traditional 3D printing methods involve the use of heat, toxic organic solvents, or toxic photoinitiators for fabrication of synthetic scaffolds. In this study, two thermoresponsive water-based biodegradable polyurethane dispersions (PU1 and PU2) were synthesized which may form gel near 37 °C without any crosslinker. The stiffness of the hydrogel could be easily fine-tuned by the solid content of the dispersion. Neural stem cells (NSCs) were embedded into the polyurethane dispersions before gelation. The dispersions containing NSCs were subsequently printed and maintained at 37 °C. The NSCs in 25-30% PU2 hydrogels (∼680-2400 Pa) had excellent proliferation and differentiation but not in 25-30% PU1 hydrogels. Moreover, NSC-laden 25-30% PU2 hydrogels injected into the zebrafish embryo neural injury model could rescue the function of impaired nervous system. However, NSC-laden 25-30% PU1 hydrogels only showed a minor repair effect in the zebrafish model. In addition, the function of adult zebrafish with traumatic brain injury was rescued after implantation of the 3D-printed NSC-laden 25% PU2 constructs. Therefore, the ne...Continue Reading

References

Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
May 1, 1996·Journal of Biomedical Materials Research·A Park, L G Cima
Dec 10, 1990·Clinical Materials·J M Schakenraad, P J Dijkstra
Nov 9, 2000·Nature·P J Horner, F H Gage
May 18, 2006·Journal of Neural Engineering·C Deister, C E Schmidt
Jun 17, 2006·Journal of Biomedical Materials Research. Part a·Katarzyna Gorna, Sylwester Gogolewski
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yun-Bi LuAndreas Reichenbach
Jan 18, 2007·Development, Growth & Differentiation·Haruki Ochi, Monte Westerfield
Jun 1, 2007·Artificial Organs·Matthias SiepeHendrik T Tevaearai
Dec 14, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·C Z LiuJ T Czernuszka
May 27, 2008·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Payam AkhyariArtur Lichtenberg
Jul 29, 2008·Biophysical Journal·Krishanu SahaKevin E Healy
Oct 16, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yi-Chao HsuIng-Ming Chiu
Oct 24, 2008·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Robert Katzman
Apr 17, 2009·Neuroreport·Wonhye LeeSeung-Schik Yoo
Mar 10, 2010·Experimental Neurology·Yeong-Bae LeeSeung-Schik Yoo
Apr 20, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Carlos C ChangJames B Hoying
May 12, 2012·Cardiology Research and Practice·Marie-Noëlle GiraudHendrik T Tevaearai
Sep 17, 2013·Advanced Materials·Jos MaldaDietmar W Hutmacher
Oct 17, 2013·The Laryngoscope·Nicholas HamiltonMartin Birchall
Sep 16, 2014·Neural Regeneration Research·Li XiaoRyoji Ide

❮ Previous
Next ❯

Citations

Oct 16, 2015·Biotechnology Letters·Guifang Gao, Xiaofeng Cui
Apr 5, 2016·Current Opinion in Biotechnology·Wei ZhuShaochen Chen
Apr 18, 2016·Drug Discovery Today·Ibrahim T OzbolatVeli Ozbolat
Apr 27, 2016·Biomaterials Research·Bon Kang GuChun-Ho Kim
May 3, 2016·Drug Discovery Today·Fábio G TeixeiraAntónio J Salgado
Dec 10, 2016·ACS Applied Materials & Interfaces·Sang-Hyun PyoShaochen Chen
Dec 21, 2016·Advanced Healthcare Materials·Haitao CuiLijie Grace Zhang
Feb 12, 2017·Journal of Biomedical Materials Research. Part a·Stephanie T BendtsenMei Wei
Apr 4, 2017·Advanced Materials·R Daniel PeddeMohsen Akbari
Apr 12, 2017·Stem Cells and Development·Karin LauschkeVanessa Jane Hall
May 26, 2017·Biotechnology Journal·Liqun Ning, Xiongbiao Chen
May 31, 2017·Advanced Healthcare Materials·Stuart KyleIain S Whitaker
Jan 21, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nhayoung HongGeunHyung Kim
Feb 8, 2018·Journal of Functional Biomaterials·Karthik Tappa, Udayabhanu Jammalamadaka
Oct 7, 2017·Pediatric Research·Chin Siang OngNarutoshi Hibino
Jan 3, 2018·Advanced Healthcare Materials·Zhaozhu ZhengDavid L Kaplan
Apr 20, 2018·Journal of Medical Engineering & Technology·Esmaeil BiazarDariush Biazar
Jan 24, 2018·Biomedical Materials·Agnes E HaggertyMartin Oudega
Nov 28, 2017·Scientific Reports·Zhengchu TanAntonio Elia Forte
Dec 6, 2017·Frontiers in Bioengineering and Biotechnology·Michaela Thomas, Stephanie M Willerth
Nov 25, 2017·Neural Regeneration Research·Hao-Wei Han, Shan-Hui Hsu
Aug 18, 2017·Chemical Society Reviews·Qiutong HuangJianzhong Du
Jan 30, 2018·Journal of Biomedical Materials Research. Part a·Jana SogorkovaVladimir Velebny
Sep 27, 2018·Biomaterials Science·Gillian Dumsile MahumaneViness Pillay
Sep 15, 2018·Tissue Engineering. Part a·Courtney M DumontLonnie D Shea
Mar 22, 2019·Tissue Engineering. Part B, Reviews·Juan M ColazoWesley P Thayer
Feb 16, 2019·Current Pharmaceutical Design· AfsanaKeerti Jain
Jul 5, 2019·Chemical Society Reviews·Laurine ValotGilles Subra
Aug 23, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Hamed AmaniHamidreza Pazoki-Toroudi
May 23, 2019·Journal of Biomedical Materials Research. Part a·Kecen XiaoQiang Fu
Jul 18, 2019·Biofabrication·Prasansha Rastogi, Balasubramanian Kandasubramanian
Sep 24, 2016·Biofabrication·Katja HölzlAleksandr Ovsianikov
Feb 25, 2020·Tissue Engineering. Part B, Reviews·Michelle GriffinDietmar Werner Hutmacher
Apr 3, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hiroyuki Tetsuka, Su Ryon Shin
May 28, 2020·Lab on a Chip·Cynthia RichardVictor J Cadarso
Aug 1, 2020·Small·Amin GhavamiNejadAli Khademhosseini
Sep 27, 2020·International Journal of Molecular Sciences·Tullio GenovaFederico Mussano
Dec 28, 2016·European Heart Journal Cardiovascular Imaging·Denisa MuraruLuigi P Badano

❮ Previous
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