3D confocal laser-scanning microscopy for large-area imaging of the corneal subbasal nerve plexus

Scientific Reports
S AllgeierBernd Köhler

Abstract

The capability of corneal confocal microscopy (CCM) to acquire high-resolution in vivo images of the densely innervated human cornea has gained considerable interest in using this non-invasive technique as an objective diagnostic tool for staging peripheral neuropathies. Morphological alterations of the corneal subbasal nerve plexus (SNP) assessed by CCM have been shown to correlate well with the progression of neuropathic diseases and even predict future-incident neuropathy. Since the field of view of single CCM images is insufficient for reliable characterisation of nerve morphology, several image mosaicking techniques have been developed to facilitate the assessment of the SNP in large-area visualisations. Due to the limited depth of field of confocal microscopy, these approaches are highly sensitive to small deviations of the focus plane from the SNP layer. Our contribution proposes a new automated solution, combining guided eye movements for rapid expansion of the acquired SNP area and axial focus plane oscillations to guarantee complete imaging of the SNP. We present results of a feasibility study using the proposed setup to evaluate different oscillation settings. By comparing different image selection approaches, we sho...Continue Reading

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Citations

Nov 14, 2019·Brain Sciences·Eric A Moulton, David Borsook
Oct 10, 2020·Journal of Refractive Surgery·Yu-Chi LiuJodhbir S Mehta
Dec 19, 2020·Journal of Neuromuscular Diseases·Lisette R M RaasingJan C Grutters
Nov 13, 2019·Current Opinion in Biotechnology·Alina Burmeister, Alexander Grünberger
Jun 3, 2021·Diagnostics·Katharina A SterenczakAngrit Stachs
Jul 9, 2021·Journal of Refractive Surgery·Stephen StewartJodhbir S Mehta
Nov 27, 2019·The Ocular Surface·Shyam Sunder TummanapalliMaria Markoulli

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

EyeGuidance App
EyeGuidance

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