3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels

Methods in Molecular Biology
Sílvia J Bidarra, Cristina C Barrias

Abstract

Three-dimensional (3D) cell culture systems have gained increasing interest among the scientific community, as they are more biologically relevant than traditional two-dimensional (2D) monolayer cultures. Alginate hydrogels can be formed under cytocompatibility conditions, being among the most widely used cell-entrapment 3D matrices. They recapitulate key structural features of the natural extracellular matrix and can be bio-functionalized with bioactive moieties, such as peptides, to specifically modulate cell behavior. Moreover, alginate viscoelastic properties can be tuned to match those of different types of native tissues. Ionic alginate hydrogels are transparent, allowing routine monitoring of entrapped cells, and crosslinking can be reverted using chelating agents for easy cell recovery. In this chapter, we describe some key steps to establish and characterize 3D cultures of mesenchymal stem cells using alginate hydrogels.

Citations

Apr 4, 2019·Nanomaterials·Moumita GhoshLihi Adler-Abramovich
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Patrícia Barros da SilvaCristina Carvalho Barrias
Apr 2, 2021·Frontiers in Bioengineering and Biotechnology·Filipa C TeixeiraSílvia J Bidarra
Jul 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qian LiYuhua Sun
Oct 14, 2021·Zygote : the Biology of Gametes and Early Embryos·Masoud HemadiMohammadreza Gholami

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