3D-cultured adipose tissue-derived stem cells inhibit liver cancer cell migration and invasion through suppressing epithelial-mesenchymal transition

International Journal of Molecular Medicine
Haihua XieJingfeng Liu

Abstract

Adipose tissue-derived stem cells (ADSCs) are considered promising candidates for stem cell therapy; however, the tumorigenicity of ADSCs remains controversial. The present study aimed to investigate the association between ADSCs and liver cancer cells, and to determine whether culture methods could influence the effects of ADSCs on liver cancer cell growth in vitro. Liver cancer cells were treated with ADSCs-conditioned medium (CM) that was collected using the two-dimensional (2D) culture method, sphere culture method, or three-dimensional (3D) culture method. After that, cell viability and apoptosis were measured using CCK-8 and Annexin V-FITC assay, respectively; the cell motility and adhesive capacity were analyzed by scratch wound healing and cell adhesion assay, respectively; the cell migration and invasion were examined by Transwell units; and the molecular mechanisms of ADSCs on effecting epithelial mesenchymal transition signaling pathway were further analyzed. The results demonstrated that ADSCs‑CM was able to inhibit the growth of liver cancer cells by inhibiting cell proliferation and promoting cell apoptosis, as well as by suppressing cell motility, adhesive capacity, migration and invasion. In addition, ADSCs‑CM w...Continue Reading

References

Apr 2, 1999·Science·M F PittengerD R Marshak
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 4, 2005·Diseases of the Colon and Rectum·Damián García-OlmoJosé Antonio Rodríguez-Montes
Jan 18, 2007·The Journal of Experimental Medicine·Marta SerafiniCatherine M Verfaillie
Feb 3, 2009·Carcinogenesis·Fabian L MuehlbergEckhard U Alt
Mar 11, 2009·Diseases of the Colon and Rectum·Damian Garcia-OlmoMaria Pascual
Sep 16, 2009·Human Pathology·Dolores López-TerradaBarbara B Knowles
Dec 3, 2010·Journal of Clinical and Experimental Hematopathology : JCEH·Kazuya SatoKeiya Ozawa
Mar 29, 2011·Journal of Cellular Physiology·Lin Li Hui TianWenjun Li
May 17, 2011·Nature·Tongbiao ZhaoYang Xu
May 21, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Akihiro SekiShuichi Kaneko
Oct 19, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lingling HouYuehui Ma
Jan 15, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Suk Ho BhangByung-Soo Kim
Jan 21, 2014·Stem Cells Translational Medicine·Brittany Daughtry, Shoukhrat Mitalipov
Jul 12, 2014·Cell and Tissue Research·Christina L RettingerKai P Leung
Sep 4, 2014·Journal of Translational Medicine·Thekkeparambil Chandrabose SrijayaNoor Hayaty Abu Kasim
Oct 12, 2014·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Exuperio Díez-TejedorBlanca Fuentes Gimeno
Aug 5, 2015·Aesthetic Surgery Journal·Peter B Fodor, Stephen G Paulseth
Aug 27, 2015·BioResearch Open Access·Ching-Ming YangShan-Hui Hsu
Oct 30, 2015·International Journal of Molecular Sciences·Nkemcho OjehOlivera Stojadinovic
Nov 12, 2015·Life Sciences·Maryam Hashemi, Fatemeh Kalalinia
Nov 21, 2015·Stem Cells and Cloning : Advances and Applications·Vaughan FeisstMichelle B Locke
Nov 26, 2015·Stem Cell Research & Therapy·Krishna M PanchalingamLeo A Behie
Nov 26, 2015·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·Kyu Joo ParkWon Ho Kim

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

ModFit LT
Image [UNK]
SPSS

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