3D electron microscopy of the interaction of kinesin with tubulin

Cell Structure and Function
K HiroseLinda A Amos

Abstract

We have studied the structure of microtubules decorated with kinesin motor domains in different nucleotide states by 3D electron microscopy. Having docked the atomic coordinates of both dimeric ADP.kinesin and tubulin heterodimer into a map of kinesin dimers bound to microtubules in the presence of ADP, we try to predict which regions of the proteins interact in the weakly binding state. When either the presence of 5'-adenylyimidodiphosphate (AMP-PNP) or an absence of nucleotides puts motor domains into a strongly-bound state, the 3D maps show changes in the motor domains which modify their interaction with beta-tubulin. The maps also show differences in beta-tubulin conformation compared with undecorated microtubules or those decorated with weakly-bound motors. Strongly-bound ncd appears to produce an identical change.

References

Jan 1, 1995·The Journal of Cell Biology·Y H Song, E Mandelkow
Jun 1, 1993·The Journal of Cell Biology·S RayJ Howard
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·K HiroseL A Amos
Feb 1, 1997·Current Opinion in Cell Biology·L A Amos, K Hirose
Jul 25, 1997·Cell·G WoehlkeR D Vale
Sep 4, 1997·Nature·U Henningsen, M Schliwa
Jan 24, 1998·Biochemistry·S SackE Mandelkow
Jan 1, 1997·Annual Review of Cell and Developmental Biology·R D Vale, R J Fletterick
Jun 10, 1998·Journal of Molecular Biology·K HiroseL A Amos
Jun 17, 1998·Nature Structural Biology·E NogalesJ Löwe
Aug 26, 1998·Science·S A Endow, K W Waligora
Oct 31, 1998·Nature·E P SablinR J Fletterick
Feb 16, 1999·Cell·A DesaiC E Walczak
Feb 16, 1999·Cell·E NogalesK H Downing

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Citations

Sep 15, 2005·Journal of Cell Science·Julia VentRichard Hallworth
Feb 3, 2006·Journal of Muscle Research and Cell Motility·Stefan Lakämper, Edgar Meyhöfer
Feb 28, 2002·Journal of Molecular Biology·Hanspeter NiederstrasserEva Nogales
Apr 8, 2000·Current Opinion in Structural Biology·L A Amos
Oct 29, 2010·Journal of the American Chemical Society·Max von DeliusDan-Tam D Tang

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