3D full-field quantification of cell-induced large deformations in fibrillar biomaterials by combining non-rigid image registration with label-free second harmonic generation

Biomaterials
Alvaro Jorge-PeñasHans Van Oosterwyck

Abstract

To advance our current understanding of cell-matrix mechanics and its importance for biomaterials development, advanced three-dimensional (3D) measurement techniques are necessary. Cell-induced deformations of the surrounding matrix are commonly derived from the displacement of embedded fiducial markers, as part of traction force microscopy (TFM) procedures. However, these fluorescent markers may alter the mechanical properties of the matrix or can be taken up by the embedded cells, and therefore influence cellular behavior and fate. In addition, the currently developed methods for calculating cell-induced deformations are generally limited to relatively small deformations, with displacement magnitudes and strains typically of the order of a few microns and less than 10% respectively. Yet, large, complex deformation fields can be expected from cells exerting tractions in fibrillar biomaterials, like collagen. To circumvent these hurdles, we present a technique for the 3D full-field quantification of large cell-generated deformations in collagen, without the need of fiducial markers. We applied non-rigid, Free Form Deformation (FFD)-based image registration to compute full-field displacements induced by MRC-5 human lung fibrobla...Continue Reading

Citations

Jan 8, 2020·PloS One·Christian SteuweHans Van Oosterwyck
Oct 29, 2018·Journal of Nanobiotechnology·Hannelore BovéMarcel Ameloot
Jun 27, 2020·Cytoskeleton·Marie-Mo VaeyensHans Van Oosterwyck
May 5, 2020·Frontiers in Bioengineering and Biotechnology·Abdel Rahman Abdel Fattah, Adrian Ranga
Aug 23, 2020·Journal of Biomechanics·Guy I WienerDaphne Weihs
Nov 16, 2018·ACS Applied Materials & Interfaces·Dina PetrovaAlbert M Brouwer
Sep 15, 2018·Journal of Chemical Theory and Computation·Silvio OsellaStefan Knippenberg

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