3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration

Acta Biomaterialia
Urszula StachewiczAsa H Barber

Abstract

The interaction between resident cells and electrospun nanofibers is critical in determining resultant osteoblast proliferation and activity in orthopedic tissue scaffolds. The use of techniques to evaluate cell-nanofiber interactions is critical in understanding scaffold function, with visualization promising unparalleled access to spatial information on such interactions. 3D tomography exploiting focused ion beam (FIB)-scanning electron microscopy (SEM) was used to examine electrospun nanofiber scaffolds to understand the features responsible for (osteoblast-like MC3T3-E1 and UMR106) cell behavior and resultant scaffold function. 3D imaging of cell-nanofiber interactions within a range of electrospun poly(d,l-lactide-co-glycolide acid) (PLGA) nanofiber scaffold architectures indicated a coherent interface between osteoblasts and nanofiber surfaces, promoting osteoblast filopodia formation for successful cell growth. Coherent cell-nanofiber interfaces were demonstrated throughout a randomly organized and aligned nanofiber network. Gene expression of UMR106 cells grown on PLGA fibers did not deviate significantly from those grown on plastic, suggesting maintenance of phenotype. However, considerably lower expression of Ibsp and...Continue Reading

References

Mar 11, 2000·Biomaterials·K Anselme
Nov 19, 2005·Science·Molly M Stevens, Julian H George
May 17, 2006·Acta Biomaterialia·Eugene D BolandGary L Bowlin
Jun 13, 2006·Nature Materials·James R WilsonScott A Barnett
Dec 16, 2006·Biotechnology and Bioengineering·Tao SunSheila MacNeil
Sep 25, 2007·Advanced Drug Delivery Reviews·Gregory C Rutledge, Sergey V Fridrikh
Mar 20, 2008·Journal of the Royal Society, Interface·Monika RumplerPeter Fratzl
May 10, 2008·Nature Reviews. Molecular Cell Biology·Pieta K Mattila, Pekka Lappalainen
Jul 22, 2008·Biomaterials·Sangamesh G KumbarCato T Laurencin
Jul 26, 2008·Nature Nanotechnology·Arkadii ArinsteinEyal Zussman
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ying LiuDev Chidambaram
Jul 31, 2010·International Journal of Cell Biology·Fei XueDavid A Knecht
Sep 14, 2010·Biointerphases·Krishna P KommareddyPeter Fratzl
Oct 29, 2010·Microscopy Research and Technique·Louise E SmithSheila Macneil
Nov 6, 2010·Journal of Microscopy·J S EarlS J Milne
Mar 5, 2011·Acta Biomaterialia·Cedryck Vaquette, Justin John Cooper-White
Apr 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jin-Woo LeeHun-Kuk Park
Apr 13, 2012·Macromolecular Bioscience·Roey TzezanaShulamit Levenberg
Apr 25, 2012·ACS Applied Materials & Interfaces·Urszula StachewiczAsa H Barber
May 25, 2012·ACS Nano·Menglin ChenFlemming Besenbacher
May 29, 2012·Nature Materials·Britta TrappmannWilhelm T S Huck
Oct 6, 2012·Biomaterials·Yen B TruongJohn A M Ramshaw
Jun 4, 2013·Micron : the International Research and Review Journal for Microscopy·Russell J BaileyAsa H Barber
Aug 3, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Nanayaa F Hughes-BrittainCees W M Bastiaansen
Jan 7, 2014·Frontiers in Bioscience (Landmark Edition)·Yiping WangWei Chen
Jul 15, 2015·Bone·Elena García-GaretaGordon W Blunn
Jul 16, 2015·ACS Applied Materials & Interfaces·Urszula StachewiczAsa H Barber

❮ Previous
Next ❯

Citations

Oct 21, 2016·Tissue Engineering. Part C, Methods·Lauren SfakisAlexander Khmaladze
Mar 30, 2018·International Journal of Molecular Sciences·Lauren SfakisJames Castracane
Jun 15, 2018·Advanced Materials·Zhaoxu TuRainer Haag
Jan 20, 2019·Journal of Tissue Engineering and Regenerative Medicine·Maya Schnabel-LubovskyDror Seliktar
Apr 10, 2019·Polymers·Piotr K SzewczykUrszula Stachewicz
Nov 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiankang HeDichen Li
Jul 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jianyong FengChangyou Gao
Jul 29, 2020·International Journal of Molecular Sciences·Heather E OwstonElena Jones
Jan 10, 2021·Colloids and Surfaces. B, Biointerfaces·Zuzanna J KrysiakUrszula Stachewicz
Jan 10, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·Itsumi YoshimotoSatoshi Imazato
Nov 24, 2020·ACS Applied Bio Materials·Zuzanna J KrysiakUrszula Stachewicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.