3D In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method

Methods in Molecular Biology
Fransisca Leonard, Biana Godin

Abstract

Tumor microenvironment composition and architecture are known as a major factor in orchestrating the tumor growth and its response to various therapies. In this context, in vivo studies are necessary to evaluate the responses. However, while tumor cells can be of human origin, tumor microenvironment in the in vivo models is host-based. On the other hand, in vitro studies in a flat monoculture of tumor cells (the most frequently used in vitro tumor model) are unable to recapitulate the complexity of tumor microenvironment. Three-dimensional (3D) in vitro cell cultures of tumor cells have been proven to be an important experimental tool in understanding mechanisms of tumor growth, response to therapeutics, and transport of nutrients/drugs. We have recently described a novel tool to create 3D co-cultures of tumor cells and cells in the tumor microenvironment. Our method utilizes magnetic manipulation/levitation of the specific ratios of tumor cells and cells in the tumor microenvironment (from human or animal origin) aiding in the formation of tumor spheres with defined cellular composition and density, as quickly as within 24 h. This chapter describes the experimental protocols developed to model the 3D structure of the cancer en...Continue Reading

Citations

Apr 12, 2016·Pharmacology & Therapeutics·Sritama Nath, Gayathri R Devi
Feb 7, 2017·Journal of Applied Toxicology : JAT·Hoay Yan CheahLip Yong Chung
Feb 29, 2020·Tissue Engineering. Part B, Reviews·Ishani D PremaratneJason A Spector
Mar 4, 2020·Advanced Healthcare Materials·Jing NieYong He
Aug 24, 2019·Tissue Engineering. Part C, Methods·Swathi SwaminathanAlisa Morss Clyne
Sep 26, 2020·Frontiers in Bioengineering and Biotechnology·Hermann B FrieboesBiana Godin
Jun 12, 2019·Scientific Reports·Nancy Elena Guzmán-DelgadoPilar Carranza-Rosales
Mar 28, 2019·Journal of Cell Communication and Signaling·Priyanka RayMohiuddin Quadir
Jun 9, 2018·Frontiers in Bioengineering and Biotechnology·Jorge A BelgodereJangwook P Jung
Oct 9, 2020·International Journal of Molecular Sciences·Mohamed Zakaria NassefMarcus Krüger
Mar 2, 2021·Applied Magnetic Resonance·Oxana TseytlinMark Tseytlin
Feb 27, 2020·Bioprinting·Y Cagri OztanEmrah Celik
Oct 16, 2020·Journal of Biomedical Materials Research. Part a·Leila RezakhaniAkram Alizadeh
Mar 11, 2019·ACS Biomaterials Science & Engineering·Priyanka RayMohiuddin Quadir
May 15, 2018·ACS Biomaterials Science & Engineering·Anna M SitarskiMichaela R Reagan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.