3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow

IUCrJ
Diana Cf MonteiroMartin Trebbin

Abstract

Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses. Furthermore, it has enabled the study of protein dynamics by the capture of atomically resolved and time-resolved molecular movies. However, the study of many biologically relevant targets is still severely hindered by high sample consumption and lengthy data-collection times. By combining serial synchrotron crystallography (SSX) with 3D printing, a new experimental platform has been created that tackles these challenges. An affordable 3D-printed, X-ray-compatible microfluidic device (3D-MiXD) is reported that allows data to be collected from protein microcrystals in a 3D flow with very high hit and indexing rates, while keeping the sample consumption low. The miniaturized 3D-MiXD can be rapidly installed into virtually any synchrotron beamline with only minimal adjustments. This efficient collection scheme in combination with its mixing geometry paves the way for recording molecular movies at synchrotrons by mixing-triggered millisecond time-resolved SSX.

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Citations

Jan 9, 2021·Quarterly Reviews of Biophysics·Marcus Fischer
Aug 28, 2020·Current Opinion in Structural Biology·Arwen R Pearson, Pedram Mehrabi
Feb 10, 2021·Acta Crystallographica. Section D, Structural Biology·Claudia StohrerArwen R Pearson
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Aug 28, 2021·Science·Gisela Brändén, Richard Neutze
Oct 5, 2021·Acta Crystallographica. Section D, Structural Biology·Diana C F MonteiroArwen R Pearson

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Methods Mentioned

BETA
X-ray
chip
chips

Software Mentioned

MOLREP
COMSOL
XGANDALF
XDS
CTRUNCATE
XFEL
MOSFLM
REFMAC
Coot
MiXD

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