3D multifocus astigmatism and compressed sensing (3D MACS) based superresolution reconstruction

Biomedical Optics Express
Jiaqing HuangJianjie Ma

Abstract

Single molecule based superresolution techniques (STORM/PALM) achieve nanometer spatial resolution by integrating the temporal information of the switching dynamics of fluorophores (emitters). When emitter density is low for each frame, they are located to the nanometer resolution. However, when the emitter density rises, causing significant overlapping, it becomes increasingly difficult to accurately locate individual emitters. This is particularly apparent in three dimensional (3D) localization because of the large effective volume of the 3D point spread function (PSF). The inability to precisely locate the emitters at a high density causes poor temporal resolution of localization-based superresolution technique and significantly limits its application in 3D live cell imaging. To address this problem, we developed a 3D high-density superresolution imaging platform that allows us to precisely locate the positions of emitters, even when they are significantly overlapped in three dimensional space. Our platform involves a multi-focus system in combination with astigmatic optics and an ℓ 1-Homotopy optimization procedure. To reduce the intrinsic bias introduced by the discrete formulation of compressed sensing, we introduced a de...Continue Reading

References

Aug 10, 2006·Nature Methods·Michael J RustXiaowei Zhuang
Aug 12, 2006·Science·Eric BetzigHarald F Hess
Sep 19, 2006·Biophysical Journal·Samuel T HessMichael D Mason
Apr 1, 2011·Nature Methods·Seamus J HoldenAchillefs N Kapanidis
May 12, 2011·Biomedical Optics Express·Fang HuangKeith A Lidke
Feb 15, 2012·Optics Express·I IzeddinJ B Sibarita
Apr 24, 2012·Nature Methods·Lei ZhuBo Huang
Jun 9, 2012·Biophysical Journal·Eran A MukamelXiaowei Zhuang
Dec 12, 2012·Nature Methods·Sara AbrahamssonMats G L Gustafsson
Feb 12, 2014·Optics Express·Hazen P BabcockXiaowei Zhuang
Jun 5, 2014·Biophysical Journal·Lusheng GuTao Xu
Jun 24, 2014·Scientific Reports·Anthony BarsicRafael Piestun
Aug 5, 2014·Optics Express·Amir TahmasbiRaimund J Ober
Nov 11, 2014·Nature Photonics·Shu JiaXiaowei Zhuang

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Citations

Sep 26, 2015·Faraday Discussions·B YangB Lounis
Nov 10, 2016·Applied Optics·Jin Li, Zilong Liu
Jul 26, 2018·Molecular Biology of the Cell·Yi-Hua ZhuJian-Qiu Wu
Jul 28, 2017·Scientific Reports·Jin LiFengdeng Liu
Oct 17, 2017·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Jizhou LiThierry Blu
Nov 25, 2018·Optics Express·Jizhou LiThierry Blu
Jun 22, 2021·Progress in Biophysics and Molecular Biology·Gianmaria CalisesiAndrea Bassi

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