3D Printed Microfluidic Probes

Scientific Reports
Ayoola BrimmoMohammad A Qasaimeh

Abstract

In this work, we fabricate microfluidic probes (MFPs) in a single step by stereolithographic 3D printing and benchmark their performance with standard MFPs fabricated via glass or silicon micromachining. Two research teams join forces to introduce two independent designs and fabrication protocols, using different equipment. Both strategies adopted are inexpensive and simple (they only require a stereolithography printer) and are highly customizable. Flow characterization is performed by reproducing previously published microfluidic dipolar and microfluidic quadrupolar reagent delivery profiles which are compared to the expected results from numerical simulations and scaling laws. Results show that, for most MFP applications, printer resolution artifacts have negligible impact on probe operation, reagent pattern formation, and cell staining results. Thus, any research group with a moderate resolution (≤100 µm) stereolithography printer will be able to fabricate the MFPs and use them for processing cells, or generating microfluidic concentration gradients. MFP fabrication involved glass and/or silicon micromachining, or polymer micromolding, in every previously published article on the topic. We therefore believe that 3D printed ...Continue Reading

References

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Citations

Aug 20, 2019·Faraday Discussions·Trevor G JohnstonAlshakim Nelson
Nov 5, 2019·Lab on a Chip·Ayoola T BrimmoMohammad A Qasaimeh
Jan 30, 2020·Nanoscale·Changyou YanXiaolong Wang
Jun 28, 2019·Analytical Methods : Advancing Methods and Applications·Benjamin T Mehl, R Scott Martin
Apr 18, 2019·Nature Communications·Pierre-Alexandre GoyetteThomas Gervais
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Pierre-Alexandre GoyetteThomas Gervais
Dec 19, 2018·Analytical Chemistry·Qiang ZhangJin-Ming Lin
May 13, 2019··Alshakim NelsonTrevor G. Johnston

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Methods Mentioned

BETA
chips
chip

Software Mentioned

COMSOL Multiphysics®
Catia
MATLAB
Zaber
Image J
GNU
ImageJ
SolidWorks

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