3D printing with peptide-polymer conjugates for single-step fabrication of spatially functionalized scaffolds

Biomaterials Science
Paula CamachoLesley W Chow

Abstract

Biodegradable polymer-based scaffolds are widely used to provide support during early stages of regeneration and can be functionalized with various chemical groups or bioactive cues to promote desired cellular behavior. However, these scaffolds are often modified post-fabrication, which can lead to undesired changes and homogeneously distributed chemistries that fail to mimic the spatial biochemical organization found in native tissues. To address these challenges, surface functionalization can be achieved by 3D printing with pre-functionalized biodegradable polymers, such as peptide-modified polymer conjugates, to control the deposition of preferred chemistries. Peptide-PCL conjugates were synthesized with the canonical cell adhesion peptide motif RGDS or its negative control RGES and 3D printed into scaffolds displaying one or both peptides. The peptides were also modified with bioorthogonal groups, biotin and azide, to visualize peptide concentration and location by labeling with complementary fluorophores. Peptide concentration on the scaffold surface increased with increasing peptide-PCL conjugate concentration added to the ink prior to 3D printing, and scaffolds printed with the highest RGDS(biotin)-PCL concentrations sho...Continue Reading

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Citations

Jan 13, 2021·Biomaterials Science·Jonathan DoroginMarian H Hettiaratchi
Mar 13, 2021·Advanced Functional Materials·Sharon FleischerGordana Vunjak-Novakovic
Jul 2, 2021·Advanced Drug Delivery Reviews·Matthew L TanClaudia Fischbach

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Methods Mentioned

BETA
nuclear magnetic resonance
scanning electron microscopy
dissection
infrared spectroscopy
PCL
fluorescence microscopy
Assay

Software Mentioned

SPSS
Fiji
ImageJ

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