3D skeletal muscle fascicle engineering is improved with TGF-β1 treatment of myogenic cells and their co-culture with myofibroblasts

PeerJ
Jessica KriegerChristopher Malcuit

Abstract

Skeletal muscle wound healing is dependent on complex interactions between fibroblasts, myofibroblasts, myogenic cells, and cytokines, such as TGF-β1. This study sought to clarify the impact of TGF-β1 signaling on skeletal muscle cells and discern between the individual contributions of fibroblasts and myofibroblasts to myogenesis when in co-culture with myogenic cells. 3D tissue-engineered models were compared to equivalent 2D culture conditions to assess the efficacy of each culture model to predictively recapitulate the in vivo muscle environment. TGF-β1 treatment and mono-/co-cultures containing human dermal fibroblasts or myofibroblasts and C2C12 mouse myoblasts were assessed in 2D and 3D environments. Three culture systems were compared: cell monolayers grown on 2D dishes and 3D tissues prepared via a self-assembly method or collagen 1-based hydrogel biofabrication. qPCR identified gene expression changes during fibroblast to myofibroblast and myoblast differentiation between culture conditions. Changes to cell phenotype and tissue morphology were characterized via immunostaining for myosin heavy chain, procollagen, and α-smooth muscle actin. Tissue elastic moduli were measured with parallel plate compression and atomic f...Continue Reading

References

Feb 24, 2001·American Journal of Physiology. Cell Physiology·R G DennisJ A Faulkner
Jan 31, 2003·The Journal of Biological Chemistry·Joshua R Sanes
Aug 2, 2003·Journal of Anatomy·Maria HillG Goldspink
Jan 13, 2004·Physiological Reviews·Sophie B P Chargé, Michael A Rudnicki
Jun 21, 2005·Nature Biotechnology·Shulamit LevenbergRobert Langer
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Nov 13, 2007·Current Opinion in Cell Biology·Fabien Le Grand, Michael A Rudnicki
Jun 7, 2008·Circulation Research·Kris Noel DahlJan Lammerding
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Joseph W SangerJean M Sanger
Nov 26, 2010·Journal of Biomechanics·Gretchen A Meyer, Richard L Lieber
Jan 22, 2011·Cells, Tissues, Organs·Tracy A GwytherMarsha W Rolle
Jun 21, 2011·Environmental Science & Technology·Hanna L Tuomisto, M Joost Teixeira de Mattos
Jun 23, 2011·Journal of Tissue Engineering and Regenerative Medicine·Marloes L P LangelaanFrank P T Baaijens
Jul 30, 2011·Skeletal Muscle·Christopher J MannPura Muñoz-Cánoves
Sep 1, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jacob KofflerShulamit Levenberg
Sep 20, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Nathan Sandbo, Nickolai Dulin
Mar 1, 2012·Journal of Tissue Engineering and Regenerative Medicine·Michael R WeistLisa M Larkin
May 1, 2012·Meat Science·Mark J Post
Jan 9, 2013·Journal of Bodywork and Movement Therapies·Andrea TurrinaCarla Stecco
Jun 6, 2013·American Journal of Physiology. Endocrinology and Metabolism·Yanjun DongLiping Zhang
Jun 14, 2013·American Journal of Physiology. Cell Physiology·Richard L Lieber, Samuel R Ward
Feb 27, 2015·EMBO Molecular Medicine·Claudia FuocoCesare Gargioli
Apr 17, 2018·Journal of Visualized Experiments : JoVE·Hannah A StrobelMarsha W Rolle

❮ Previous
Next ❯

Citations

Oct 28, 2019·NPJ Science of Food·Luke A MacQueenKevin Kit Parker
May 22, 2021·Food Science of Animal Resources·Tae Kyung HongSung Gu Han

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
AFM

Software Mentioned

MYOG
MyoCon
PrimerBlast
3D
CoCon
Microsquisher
ImageJ
MATLAB
GraphPad
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.