Jul 15, 2016

3D sorghum reconstructions from depth images enable identification of quantitative trait loci regulating shoot architecture

BioRxiv : the Preprint Server for Biology
Ryan F. McCormickJohn E. Mullet

Abstract

Dissecting the genetic basis of complex traits is aided by frequent and non-destructive measurements. Advances in range imaging technologies enable the rapid acquisition of three-dimensional (3D) data from an imaged scene. A depth camera was used to acquire images of Sorghum bicolor, an important grain, forage, and bioenergy crop, at multiple developmental timepoints from a greenhouse-grown recombinant inbred line population. A semi-automated software pipeline was developed and used to generate segmented, 3D plant reconstructions from the images. Automated measurements made from 3D plant reconstructions identified quantitative trait loci (QTL) for standard measures of shoot architecture such as shoot height, leaf angle and leaf length, and for novel composite traits such as shoot compactness. The phenotypic variability associated with some of the QTL displayed differences in temporal prevalence; for example, alleles closely linked with the sorghum Dwarf3 gene, an auxin transporter and pleiotropic regulator of both leaf inclination angle and shoot height, influence leaf angle prior to an effect on shoot height. Furthermore, variability in composite phenotypes that measure overall shoot architecture, such as shoot compactness, is...Continue Reading

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Mentioned in this Paper

Computer Software
Quantitative Trait Loci
Sorghum bicolor
PLRG1
Genes
Three-dimensional
Auxins
Inbred Strain
Avian Crop
Genetic Pleiotropy

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