3D + time blood flow mapping using SPIM-microPIV in the developing zebrafish heart

Biomedical Optics Express
Vytautas Zickus, Jonathan M Taylor

Abstract

We present SPIM-μPIV as a flow imaging system, capable of measuring in vivo flow information with 3D micron-scale resolution. Our system was validated using a phantom experiment consisting of a flow of beads in a 50 μm diameter FEP tube. Then, with the help of optical gating techniques, we obtained 3D + time flow fields throughout the full heartbeat in a ∼3 day old zebrafish larva using fluorescent red blood cells as tracer particles. From this we were able to recover 3D flow fields at 31 separate phases in the heartbeat. From our measurements of this specimen, we found the net pumped blood volume through the atrium to be 0.239 nL per beat. SPIM-μPIV enables high quality in vivo measurements of flow fields that will be valuable for studies of heart function and fluid-structure interaction in a range of small-animal models.

References

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Citations

Sep 29, 2019·Nature Methods·Venkatakaushik VoletiElizabeth M C Hillman
May 9, 2019·Biomedical Optics Express·Yongzhuang ZhouAndrew R Harvey
Oct 12, 2021·Physical Biology·María F SampedroValeria Sigot

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence imaging
transgenic
light sheet microscopy

Software Mentioned

μPIV
ImageJ
SPIM
OpenPIV
BF

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