PMID: 9173907Apr 1, 1997Paper

3Fe-4S] <--> [4Fe-4S] cluster interconversion in Desulfovibrio africanus ferredoxin III: properties of an Asp14 --> Cys mutant

The Biochemical Journal
J L BuschA J Thomson

Abstract

The 8Fe ferredoxin III from Desulfovibrio africanus is a monomeric protein which contains two [4Fe-4S]2+/1+ clusters, one of which is labile and can readily and reversibly lose one Fe under oxidative conditions to yield a [3Fe-4S]1+/0 cluster. This 4Fe cluster has an S = 3/2 ground sping state insteaed of S = 1/2 in the reduced +1 state [George, Armstrong, Hatchikian and Thomson (1989) Biochem. J. 264, 275-284]. The co-ordination to this cluster is unusual in that an aspartate (Asp14, D14, is found where a cysteine residue normally occurs. Using a mutant protein obtained from the overexpression in Escherichia coli of a synthetic gene in which Asp14, the putative ligand to the removable Fe, has been changed to Cys, we have studied the cluster interconversion properties of the labile cluster. Analysis by EPR and magnetic-circular-dichroism spectroscopies showed that the Asp14 --> Cys (D14C) mutant contains two [4Fe-4S]2+/1+ clusters, both with S = 1/2 in the reduced state. Also, unlike in native 8Fe D. africanus ferredoxin III, the 4Fe <--> 3Fe cluster interconversion reaction was found to be sluggish and did not go to completion. It is inferred that the reversibility of the reaction in the native protein is due to the presence o...Continue Reading

Citations

Nov 22, 2008·Biochemical Society Transactions·Jason C CrackNick E Le Brun
Apr 13, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Monika Nøhr LøvgreenPernille Harris
Apr 6, 2018·Chemical Science·Ma Teresa Pellicer MartinezNick E Le Brun
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jason C CrackAndrew J Thomson
Jan 14, 2012·Antioxidants & Redox Signaling·Jason C CrackNick E Le Brun
Jan 9, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Maja MarticHans Erik Mølager Christensen
Apr 21, 2017·Nature Communications·Anne VolbedaJuan C Fontecilla-Camps
Apr 22, 2009·The Journal of Physical Chemistry. a·Shuqiang Niu, Toshiko Ichiye
Jul 17, 1998·Current Opinion in Chemical Biology·M K Johnson
Dec 10, 2016·Chemical Reviews·R H Holm, Wayne Lo
May 24, 2011·Bioelectrochemistry·Raquel GrazinaJosé J G Moura

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