PMID: 6406256Jun 1, 1983Paper

3H]lysine incorporation into protein of hemisected, cycloheximide-treated spinal cord

Experimental Neurology
J J Bernstein, M R Wells

Abstract

A single application of a protein synthesis inhibitor, puromycin, in the lesion site of a spinal hemisection transiently decreases protein incorporation for less than 24 h and results in axonal sprouting and dendritic and synaptic stability for at least 60 days. In this study we characterized the protein-altering properties of single applications of a different protein synthesis inhibitor, cycloheximide, for comparison. The protein incorporation of [3H] lysine into hemisected rat spinal cord (left side, T2) treated with cycloheximide (100 micrograms/ml in Gelfoam sponge placed in wound at time of lesion) was tested 3 h, 6 h, 12 h, 1, 3, 7, and 14 days later and compared with that in 0.9% saline in Gelfoam implants as normal controls (N = 54). One hour prior to utilization, the animals were injected subcutaneously with 200 mu Ci L-[4,5(n)-3H]lysine monohydrochloride. No treatment-related differences in brain radioactivity were noted at any time postoperation. In spinal cord, inhibition of amino acid uptake by cycloheximide was not demonstrable at 3 h after hemisection, but could be detected from 6 h to 1 day after implantation. During that time, the TCA-soluble radioactivity at the lesion site was greater than in control animals...Continue Reading

References

Apr 1, 1978·Journal of Neurocytology·J J BernsteinM E Bernstein
Nov 1, 1977·Experimental Neurology·M E Bernstein, J J Bernstein
Jan 1, 1973·The International Journal of Neuroscience·M E Bernstein, J J Bernstein
Jan 1, 1973·Brain, Behavior and Evolution·J J Bernstein, M E Bernstein
Aug 1, 1973·Journal of the Neurological Sciences·S C Bondy
Aug 23, 1974·Brain Research·S C Bondy, J L Purdy
Jan 1, 1970·International Review of Neurobiology·S H Barondes
Jan 1, 1971·Brain : a Journal of Neurology·J Olesen
Nov 1, 1965·The American Journal of Medicine·I H Goldberg
May 1, 1971·Experimental Neurology·S C Bondy, B S Morelos
Jan 1, 1980·Progress in Brain Research·J J Bernstein, M R Wells

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