3pK, a novel mitogen-activated protein (MAP) kinase-activated protein kinase, is targeted by three MAP kinase pathways.

Molecular and Cellular Biology
S LudwigU R Rapp

Abstract

Recently we have identified a mitogen-activated protein kinase (MAPK)-activated protein kinase, named 3pK (G. Sithanandam, F. Latif, U. Smola, R. A. Bernal, F.-M. Duh, H. Li, I. Kuzmin, V. Wixler, L. Geil, S. Shresta, P. A. Lloyd, S. Bader, Y. Sekido, K. D. Tartof, V. I. Kashuba, E. R. Zabarovsky, M. Dean, G. Klein, B. Zbar, M. I. Lerman, J. D. Minna, U. R. Rapp, and A. Allikmets, Mol. Cell. Biol. 16:868-876, 1996). In vitro characterization of the kinase revealed that 3pK is activated by ERK. It was further shown that 3pK is phosphorylated in vivo after stimulation of cells with serum. However, the in vivo relevance of this observation in terms of involvement of the Raf/MEK/ERK cascade has not been established. Here we show that 3pK is activated in vivo by the growth inducers serum and tetradecanoyl phorbol acetate in promyelocytic HL60 cells and transiently transfected embryonic kidney 293 cells. Activation of 3pK was Raf dependent and was mediated by the Raf/MEK/ERK kinase cascade. 3pK was also shown to be activated after stress stimulation of cells. In vitro studies with recombinant proteins demonstrate that in addition to ERK, members of other subgroups of the MAPK family, namely, p38RK and Jun-N-terminal kinases/stress-ac...Continue Reading

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Citations

Jan 19, 2016·Frontiers in Cell and Developmental Biology·Matthias Gaestel
Feb 12, 2009·Gastroenterology·Hironobu TsukadaKazuaki Chayama
Aug 11, 2004·Experimental Cell Research·Vera ZakowskiStephan Ludwig
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Dec 21, 2010·Biochimica Et Biophysica Acta·Alexander PlotnikovRony Seger
Apr 26, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Majid Ghayour-MobarhanGordon A A Ferns
Apr 9, 2015·PloS One·Peggy PrickaertsJan Willem Voncken
May 25, 2002·British Journal of Haematology·Giorgio Lambertenghi DeliliersDavide Soligo
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·O WerzO Rådmark
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Nov 28, 2002·Biological Chemistry·Yu Shi, Matthias Gaestel
Nov 26, 2004·The Journal of Biological Chemistry·Jan Willem VonckenUlf R Rapp

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