4-Chloro-alpha-cyanocinnamic acid is an advanced, rationally designed MALDI matrix.

Proceedings of the National Academy of Sciences of the United States of America
Thorsten JaskollaM Karas

Abstract

Matrix-assisted laser desorption ionization (MALDI) has become an enabling technology for the fields of protein mass spectrometry (MS) and proteomics. Despite its widespread use, for example, in protein identification via peptide mass fingerprinting, a comprehensive model for the generation of free gas-phase ions has not yet been developed. All matrices in use today, such as alpha-cyano-4-hydroxycinnamic acid (CHCA), have been found empirically and stem from the early days of MALDI. By systematic and targeted variation of the functional groups of the alpha-cyanocinnamic acid core unit, 4-chloro-alpha-cyanocinnamic acid (Cl-CCA) was selected and synthesized, and it exhibited outstanding matrix properties. Key features are a substantial increase in sensitivity and a considerably enhanced peptide recovery in proteomic analyses because of a much more uniform response to peptides of different basicity. Using Cl-CCA as a matrix for a 1 fmol bovine serum albumin (BSA) in-solution digest, the sequence coverage is raised to 48%, compared with 4% for CHCA. For a gel band containing 25 fmol of BSA, unambiguous protein identification becomes possible with Cl-CCA. These findings also imply ion formation via a chemical ionization mechanism w...Continue Reading

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Citations

Oct 16, 2012·Analytical and Bioanalytical Chemistry·Marcel WiegelmannKlaus Dreisewerd
Feb 23, 2012·Experimental Brain Research·W Volknandt, M Karas
Jul 14, 2010·Amino Acids·Bjoern MeyerMichael Karas
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Thorsten W Jaskolla, Michael Karas
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Matthew T OlsonAlfred L Yergey
Aug 15, 2013·Journal of the American Society for Mass Spectrometry·Jens SoltwischKlaus Dreisewerd
Feb 10, 2009·Journal of the American Society for Mass Spectrometry·Thorsten JaskollaJürgen Schiller
Mar 21, 2009·Journal of the American Society for Mass Spectrometry·Thorsten W JaskollaKarsten Reihs
Jul 31, 2013·Analytical Chemistry·Xiaodong WangChristoph H Borchers
Dec 12, 2013·Analytical Chemistry·Rainer CramerThorsten W Jaskolla
Aug 15, 2009·Bioconjugate Chemistry·Gang ShenJohn-Stephen A Taylor
Aug 31, 2012·Journal of Agricultural and Food Chemistry·Cosima Damiana CalvanoCarlo Zambonin
Sep 3, 2013·Journal of Agricultural and Food Chemistry·Florian BaumMonika Pischetsrieder
Jan 10, 2013·Journal of Proteome Research·Maja Pučić BakovićManfred Wuhrer
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rohit ShroffAles Svatos
Jan 1, 2009·Molecular & Cellular Proteomics : MCP·Benjamin RietschelAnsgar Poetsch
Jan 18, 2011·Applied and Environmental Microbiology·Sebastian W FuchsKarl-Dieter Entian
Mar 22, 2014·Analytical and Bioanalytical Chemistry·Klaus Dreisewerd
Jun 11, 2014·Journal of the American Society for Mass Spectrometry·Richard Knochenmuss
Apr 30, 2016·Journal of the American Society for Mass Spectrometry·Yin-Hung LaiYi-Sheng Wang
Aug 11, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroko KasaiMasayoshi Tsubuki
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Marcel WiegelmannJens Soltwisch
Dec 3, 2016·Analytical and Bioanalytical Chemistry·Suparna TambeHans-Dieter Junker
Jul 22, 2014·Journal of Mass Spectrometry : JMS·Petra HorkáJosef Cvačka
Jun 22, 2017·Analytical Chemistry·Marcel NiehausKlaus Dreisewerd
Jul 12, 2013·European Journal of Mass Spectrometry·Stephan R FagererRenato Zenobi
Nov 21, 2017·SLAS Discovery·Dale S Cornett, Michael D Scholle
Feb 11, 2014·Applied and Environmental Microbiology·Hedwig-Annabell SchildBernd Grünewald
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