PMID: 12018247May 23, 2002

4-chlorophenol degradation by chloroperoxidase from Caldariomyces fumago: formation of insoluble products

Applied Biochemistry and Biotechnology
Camilo E La Rotta, Elba P S Bon

Abstract

This study investigated the degradation of 4-chlorophenol (4-CP) by Caldariomyces fumago chloroperoxidase (CPO). Enzymatic oxidations were studied in reaction mixtures at pH 3.0, 4.0, and 6.0 in the presence and absence of Cl- containing 3.5 IU of CPO and 4-CP and hydrogen peroxide concentrations within the range of 0.5-50 and 0.005-50 mM, respectively. Distinct patterns of products regarding color, concentration, and solubility were observed. Reaction mixtures at pH 6.0 containing 3.5 IU of CPO and 5.0 mM 4-CP and H2O2 (1:1 stoichiometry) showed the highest 4-CP removal of 95% and the highest formation of a dark precipitate.

Related Concepts

4-chlorophenol, titanium (+4) salt
Archaea
Bioremediation
Chloride Peroxidase
Chlorophenols
Fermentation
Oxydol

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