Abstract
In the present study, we examined the ability of a chemically synthesized compound based on the structure of leonurine, a phytochemical component of Herba leonuri, to protect H9c2 rat ventricular cells from apoptosis induced by hypoxia and serum deprivation, as a model of ischemia. The results revealed a concentration-dependent increase in cell viability associated with leonurine treatment, accompanied by a consistent decline in lactate dehydrogenase leakage into the culture medium. The fraction of annexin V-fluorescein isothiocyanate-positive cells was increased by hypoxia but reduced by leonurine. These changes were associated with increased expression of the antiapoptotic gene, Bcl-2, and reduced expression of the proapoptotic gene, Bax. Leonurine also reduced the cytosolic Ca overload induced by hypoxia. These results suggest that leonurine elicits potent cardioprotective effects in H9c2 cells, and these effects may be mediated by inhibition of intracellular Ca overload and apoptosis during hypoxia.
References
Jan 1, 1976·The American Journal of Chinese Medicine·S Hu
Jan 1, 1976·The American Journal of Chinese Medicine·Y C KongC H Yeung
Dec 11, 1992·Circulation Research·J P CarrozzaJ P Morgan
Jul 1, 1992·Circulation·J A Bittl, T A Sanborn
Jul 1, 1992·Hypertension·J I Goldhaber, J N Weiss
Sep 1, 1991·Circulation Research·F T ThandroyenL M Buja
Jan 1, 1991·Circulation·S IlicetoP Rizzon
Jan 1, 1990·Annual Review of Physiology·M Tani
Oct 1, 1994·The Journal of Clinical Investigation·R A GottliebR L Engler
Feb 21, 1997·Science·R M KluckD D Newmeyer
Jan 1, 1997·Annual Review of Medicine·C M Rudin, C B Thompson
Feb 28, 1998·Journal of Molecular and Cellular Cardiology·S Mochizuki, K T MacLeod
Jul 27, 1999·Circulation·R SoRelle
Sep 4, 1999·Circulation Research·S BialikR N Kitsis
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R D RakhitM S Marber
Aug 30, 2000·Cardiovascular Research·N S DhallaN Makino
Feb 24, 2001·Life Sciences·C X Chen, C Y Kwan
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·P KorgeJ N Weiss
Jun 20, 2001·The Journal of Biological Chemistry·Z PanJ Ma
Feb 28, 2002·Circulation·Gregory K AsimakisCam Patterson
Jul 3, 2002·Circulation·Johan E P Waktare
Mar 5, 2004·Biochimica Et Biophysica Acta·Vladimir KirkinMartin Zörnig
Apr 27, 2005·Life Sciences·Jian SunYi Zhun Zhu
May 18, 2005·Cardiovascular Research·Vanessa P M van EmpelLeon J De Windt
Jul 19, 2005·Biochemical and Biophysical Research Communications·Koichi UemuraKen-ichi Yoshida
Nov 18, 2005·European Journal of Pharmacology·Mi Jeong KimYi-Sook Jung
Apr 25, 2006·Free Radical Biology & Medicine·Anisa JahangiriEdward J McMurchie
Jun 13, 2006·Biochemical and Biophysical Research Communications
Citations
Jul 20, 2010·Amino Acids·Qi-Hai GongYi-Zhun Zhu
Jan 29, 2013·European Journal of Medicinal Chemistry·Chen ZhongXun Sun
Oct 19, 2011·Clinical and Experimental Pharmacology & Physiology·Xin H LiuYi Z Zhu
Aug 7, 2012·Biological & Pharmaceutical Bulletin·Hong XinXue Mei Zhang
Apr 29, 2014·TheScientificWorldJournal·Qing ZhuYizhun Zhu
Feb 20, 2014·DNA and Cell Biology·Yan ZhaoLi-Ying Hao
Nov 30, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Qing LiuDaping Fan
Nov 7, 2012·Free Radical Biology & Medicine·Xin-Hua LiuYi-Zhun Zhu
Apr 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Li-Long PanXin-Hua Liu
Mar 19, 2014·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bo TanYizhun Zhu
Jan 12, 2013·European Journal of Pharmacology·Chunhua LiuYizhun Zhu
Feb 7, 2012·European Journal of Pharmacology·Xin Hua LiuYi Zhun Zhu
Oct 25, 2016·Life Sciences·Shu JiangTiejun Li
Jun 27, 2013·International Journal of Molecular Sciences·Yaqi ShenYizhun Zhu
Nov 13, 2018·Journal of Cellular Physiology·Zhi-Chao HuWen-Fei Ni
Dec 9, 2010·Biological & Pharmaceutical Bulletin·Jia QiYi Zhun Zhu
Apr 30, 2019·Acta Biochimica Et Biophysica Sinica·Yunyun LiMeirong Du
Sep 2, 2011·Biomedical Chromatography : BMC·Qing ZhuYizhun Zhu
May 10, 2019·Chinese Journal of Integrative Medicine·Yun-Yun LiMei-Rong Du
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Qiu-Yan ZhangYi-Zhun Zhu
May 31, 2018·Molecular Medicine Reports·Lin XuHongling Zhu
Dec 11, 2020·Brain and Behavior·Lu HuangYu-Ping Tang
Mar 3, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mengmeng GongYidong Wei
Jan 18, 2018·Phytochemistry·Rui-Han ZhangWei-Lie Xiao
Jun 29, 2021·ACS Chemical Neuroscience·Feng LiWenbao Li