4-Guanidino-n-butyl syringate (Leonurine, SCM 198) protects H9c2 rat ventricular cells from hypoxia-induced apoptosis

Journal of Cardiovascular Pharmacology
Xinhua LiuYi-Zhun Zhu

Abstract

In the present study, we examined the ability of a chemically synthesized compound based on the structure of leonurine, a phytochemical component of Herba leonuri, to protect H9c2 rat ventricular cells from apoptosis induced by hypoxia and serum deprivation, as a model of ischemia. The results revealed a concentration-dependent increase in cell viability associated with leonurine treatment, accompanied by a consistent decline in lactate dehydrogenase leakage into the culture medium. The fraction of annexin V-fluorescein isothiocyanate-positive cells was increased by hypoxia but reduced by leonurine. These changes were associated with increased expression of the antiapoptotic gene, Bcl-2, and reduced expression of the proapoptotic gene, Bax. Leonurine also reduced the cytosolic Ca overload induced by hypoxia. These results suggest that leonurine elicits potent cardioprotective effects in H9c2 cells, and these effects may be mediated by inhibition of intracellular Ca overload and apoptosis during hypoxia.

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Citations

Oct 19, 2011·Clinical and Experimental Pharmacology & Physiology·Xin H LiuYi Z Zhu
Aug 7, 2012·Biological & Pharmaceutical Bulletin·Hong XinXue Mei Zhang
Nov 30, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Qing LiuDaping Fan
Nov 7, 2012·Free Radical Biology & Medicine·Xin-Hua LiuYi-Zhun Zhu
Apr 12, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Li-Long PanXin-Hua Liu
Mar 19, 2014·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Bo TanYizhun Zhu
May 10, 2019·Chinese Journal of Integrative Medicine·Yun-Yun LiMei-Rong Du
Dec 13, 2019·Oxidative Medicine and Cellular Longevity·Qiu-Yan ZhangYi-Zhun Zhu
Mar 3, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mengmeng GongYidong Wei

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