PMID: 11322873Apr 27, 2001Paper

4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB. A novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds

European Journal of Biochemistry
Nanne M KamerbeekDick B Janssen

Abstract

A novel flavoprotein that catalyses the NADPH-dependent oxidation of 4-hydroxyacetophenone to 4-hydroxyphenyl acetate, was purified to homogeneity from Pseudomonas fluorescens ACB. Characterization of the purified enzyme showed that 4-hydroxyacetophenone monooxygenase (HAPMO) is a homodimer of approximately 140 kDa with each subunit containing a noncovalently bound FAD molecule. HAPMO displays a tight coupling between NADPH oxidation and substrate oxygenation. Besides 4-hydroxyacetophenone a wide range of other acetophenones are readily converted via a Baeyer-Villiger rearrangement reaction into the corresponding phenyl acetates. The P. fluorescens HAPMO gene (hapE) was characterized. It encoded a 640 amino-acid protein with a deduced mass of 71 884 Da. Except for an N-terminal extension of approximately 135 residues, the sequence of HAPMO shares significant similarity with two known types of Baeyer-Villiger monooxygenases: cyclohexanone monooxygenase (27-33% sequence identity) and steroid monooxygenase (33% sequence identity). The HAPMO sequence contains several sequence motifs indicative for the presence of two Rossman fold domains involved in FAD and NADPH binding. The functional role of a recently identified flavoprotein se...Continue Reading

References

Jan 1, 1992·Annual Review of Microbiology·S HarayamaE L Neidle
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Feb 1, 1988·Journal of Bacteriology·Y C ChenC T Walsh
Oct 1, 1987·Applied and Environmental Microbiology·L Kröckel, D D Focht
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
May 25, 1982·Biochemistry·C C RyersonC Walsh
Apr 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B Entsch, W J van Berkel
Jun 1, 1994·Applied and Environmental Microbiology·K H JonesD J Hopper
Aug 1, 1993·Applied and Environmental Microbiology·J Havel, W Reineke
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 12, 1998·Protein Science : a Publication of the Protein Society·M H EppinkW J Van Berkel
Dec 12, 1997·European Journal of Biochemistry·D BeckerJ R Andreesen
Apr 16, 1998·Trends in Biochemical Sciences·M StehrP Macheroux
Sep 24, 1999·Journal of Molecular Biology·M H EppinkW J Van Berkel
Nov 24, 1999·The Journal of Biological Chemistry·W A SuskeH P Kohler
Dec 22, 1999·Archives of Biochemistry and Biophysics·J K SuhJ D Robertus
Dec 1, 1990·Applied and Environmental Microbiology·F K Higson, D D Focht

❮ Previous
Next ❯

Citations

May 9, 2002·FEBS Letters·Marco W FraaijeDick B Janssen
Jan 7, 2003·Applied and Environmental Microbiology·Nanne M KamerbeekDick B Janssen
Aug 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Enrico MalitoAndrea Mattevi
Dec 16, 2004·Applied Microbiology and Biotechnology·Marco W FraaijeDick B Janssen
Aug 23, 2008·Applied Microbiology and Biotechnology·Anett Kirschner, Uwe T Bornscheuer
Jan 12, 2017·Chembiochem : a European Journal of Chemical Biology·Felix Martin FerroniDiederik Johannes Opperman
Aug 4, 2018·Frontiers in Microbiology·Alejandro Gran-ScheuchMarco W Fraaije
Jun 5, 2020·The Biochemical Journal·Florence MusJohn W Peters
Jul 29, 2005·The Journal of Biological Chemistry·Robert H H van den HeuvelAlbert J R Heck
Oct 12, 2010·Chembiochem : a European Journal of Chemical Biology·Gonzalo de GonzaloMarco W Fraaije
Jun 6, 2014·Applied Microbiology and Biotechnology·Nur Hazlin Hazrin-ChongMike Manefield
Apr 10, 2012·Bioscience, Biotechnology, and Biochemistry·Yoshiki TanihataKiyofumi Maruyama
Jul 28, 2015·Journal of Cellular Biochemistry·Dipendrasinh JadejaJagdeep Kaur
May 13, 2018·Applied and Environmental Microbiology·Yan ZhangJian-He Xu
Jul 20, 2005·Chemical Communications : Chem Comm·Gonzalo de GonzaloMarco W Fraaije
Sep 1, 2006·Applied Microbiology and Biotechnology·Anett KirschnerUwe T Bornscheuer
Mar 14, 2007·Journal of Bacteriology·Christina OnacaJosef Altenbuchner
Feb 19, 2010·Organic & Biomolecular Chemistry·Cristina RodríguezVicente Gotor
May 22, 2004·European Journal of Biochemistry·Nanne M KamerbeekDick B Janssen
May 27, 2008·Journal of Bacteriology·Mariëlle J H MoonenWillem J H van Berkel
Aug 13, 2013·BioMed Research International·Francisco J Enguita, Ana Lúcia Leitão
Oct 2, 2009·Chembiochem : a European Journal of Chemical Biology·Daniel E Torres PazmiñoMarco W Fraaije
Jul 30, 2016·PloS One·Felix Martin FerroniDiederik Johannes Opperman
Mar 31, 2019·Applied and Environmental Microbiology·Yuan-Yang LiuGao-Wei Zheng
Nov 12, 2003·The Journal of Biological Chemistry·Marco W FraaijeDick B Janssen
May 10, 2016·Chembiochem : a European Journal of Chemical Biology·Yohei KatsuyamaYasuo Ohnishi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.