4-Hydroxyisoleucine production of recombinant Corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation

Applied Microbiology and Biotechnology
Feng ShiHuimin Fang

Abstract

4-Hydroxyisoleucine (4-HIL) is a nonproteinogenic amino acid that exhibits insulinotropic biological activity. Here, L-isoleucine dioxygenase gene (ido) derived from Bacillus thuringiensis YBT-1520 was cloned and expressed in an L-isoleucine-producing strain, Corynebacterium glutamicum ssp. lactofermentum SN01, in order to directly convert its endogenous L-isoleucine (Ile) into 4-HIL through single-step fermentation. The effects of corn steep liquor limitation as well as ido and truncated idoΔ6 overexpression on 4-HIL production were researched. 4-HIL production by ido-overexpressing strain was improved to 65.44 ± 2.27 mM after fermented for 144 h under corn steep liquor-subsufficient condition, obviously higher than that under corn steep liquor-rich and insufficient conditions. The conversion ratio of Ile to 4-HIL increased to 0.85 mol/mol. In addition, 4-HIL production by ido-overexpressing strain was higher than that by idoΔ6-overexpressing strain, in accord with the relatively higher affinity of Ido as compared to IdoΔ6. This research generated a novel system for 4-HIL de novo biosynthesis and demonstrated corn steep liquor limitation as a useful strategy for improving 4-HIL production in recombinant C. glutamicum ssp. lact...Continue Reading

References

Oct 12, 1999·The American Journal of Physiology·C BrocaG Ribes
Mar 10, 2000·European Journal of Pharmacology·C BrocaG Ribes
May 14, 2003·Applied Microbiology and Biotechnology·M Ikeda, S Nakagawa
Apr 15, 2004·American Journal of Physiology. Endocrinology and Metabolism·Christophe BrocaMohammed Taouis
Oct 26, 2005·Bioorganic & Medicinal Chemistry Letters·Tadigoppula NarenderRamesh Chandra
Dec 13, 2006·Applied and Environmental Microbiology·Yoko AsakuraTsuyoshi Nakamatsu
Feb 13, 2007·Applied and Environmental Microbiology·Bastian BlombachBernhard J Eikmanns
Oct 22, 2009·Applied Microbiology and Biotechnology·Jin Hwan Park, Sang Yup Lee
Oct 24, 2009·Biochemical and Biophysical Research Communications·Tomohiro KoderaSakayu Shimizu
Feb 9, 2010·Natural Product Research·A B SinghArvind Kumar Srivastava
Jul 29, 2010·Applied Microbiology and Biotechnology·Sergey V SmirnovSakayu Shimizu
Aug 9, 2011·Applied and Environmental Microbiology·Makoto HibiJun Ogawa
Mar 9, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Mohammed R HaeriKenneth N White
Oct 20, 2012·Sub-cellular Biochemistry·Takashi HirasawaHiroshi Shimizu
Aug 12, 2014·Molecular and Cellular Endocrinology·Chandan Kumar MauryaAkhilesh Kumar Tamrakar

❮ Previous
Next ❯

Citations

May 7, 2016·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Mohammad Ishraq Zafar, Feng Gao
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
Feb 12, 2021·World Journal of Microbiology & Biotechnology·Yota Tsuge, Hiroki Matsuzawa
Feb 14, 2019·Journal of the American Chemical Society·Madison DavidsonWei-Chen Chang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.