4-Hydroxynonenal inhibits telomerase activity and hTERT expression in human leukemic cell lines

Free Radical Biology & Medicine
Stefania PizzimentiGiuseppina Barrera

Abstract

4-Hydroxynonenal (HNE), produced during oxidative stress, has an antiproliferative/differentiative effect in several tumor cells. Recently, it has been observed that oxidative stress accelerates telomere loss. The length of telomeres depends on the telomerase activity, and the catalytic subunit of telomerase (hTERT) is strongly up-regulated in most human cancers and inhibited by differentiating agents. In this paper the inhibitory effect of HNE on telomerase activity and hTERT expression in three human leukemic cell lines (HL-60, U937, ML-1) is reported. To investigate the molecular mechanism involved in hTERT down-regulation by HNE, the expression of several transcription factors was also studied: in all these cell lines, c-Myc was inhibited, Mad-1 was up-regulated, and Sp-1 was not affected. Moreover, in p53 wild-type ML-1 cells, HNE up-regulated p53 expression. In HL-60 cells, DNA binding activity of c-Myc and Mad-1 to the E-box sequence of the hTERT promoter was inhibited and up-regulated, respectively. In summary, HNE inhibits telomerase activity via decreased hTERT promoter activity, by modulating c-Myc/Mad-1 transcription factor expression.

References

Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Wolf, V Rotter
Oct 18, 1972·Nature: New Biology·J D Watson
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·Q ChenB N Ames
Aug 30, 1994·Biochemical and Biophysical Research Communications·G BarreraM U Dianzani
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Q Chen, B N Ames
Jan 29, 1993·Biochemical and Biophysical Research Communications·H KozutsumiH Hirai
Jan 1, 1993·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M J TuijlR Van Wijk
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·H W SharmaR Narayanan
Jun 1, 1996·Molecular and Cellular Biology·S E HoltJ W Shay
Jun 5, 1996·Biochemical and Biophysical Research Communications·G BarreraR A Canuto
Jan 1, 1996·Annual Review of Biochemistry·C W Greider
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·T M HagenB N Ames
Jun 27, 1997·Cell·M L AvantaggiatiK Kelly
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J W Shay, S Bacchetti
Nov 20, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Schreiber-Agus, R A DePinho
Feb 13, 1999·Nature Genetics·K J WuR Dalla-Favera
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·B HerbertD R Corey
Feb 8, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·T YajimaN Watanabe
Sep 12, 2000·Current Hypertension Reports·R M Touyz
Mar 7, 2001·Antioxidants & Redox Signaling·M ParolaM U Dianzani
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D XuM Henriksson
Oct 3, 2001·Journal of Hepatology·M Parola, G Robino
Feb 19, 2002·Oncogene·Anne-Lyse DucrestMarkus Nabholz
Jun 11, 2002·Biochemical and Biophysical Research Communications·N CalonghiL Masotti
Jul 13, 2002·Trends in Biochemical Sciences·Thomas von Zglinicki

❮ Previous
Next ❯

Citations

May 6, 2009·Annals of Hematology·E Miri-MoghaddamZ S Soheili
Nov 8, 2007·Nucleic Acids Research·João F PassosThomas von Zglinicki
Nov 17, 2010·Biochemistry. Biokhimii︠a︡·E E Dubinina, V A Dadali
Aug 30, 2014·Nucleic Acids Research·Jolanta CzerwińskaElżbieta Speina
Jan 5, 2013·Mutation Research·Evelyn LamyVolker Mersch-Sundermann
May 15, 2012·Free Radical Biology & Medicine·Rajendra SharmaYogesh C Awasthi
Nov 27, 2007·Molecular Aspects of Medicine·Giuseppina BarreraMario Umberto Dianzani
Sep 8, 2007·Biochimie·Anne De CianJean-Louis Mergny
Nov 24, 2007·Biochemical Pharmacology·Charis CostopoulosMartin Bennett
Oct 3, 2009·Journal of Pineal Research·K Gonca AkbulutHakan Akbulut
Jul 8, 2014·Oxidative Medicine and Cellular Longevity·Antonio AyalaSandro Argüelles
Jan 20, 2012·Free Radical Research·Alicja WinczuraBarbara Tudek
Dec 27, 2013·Oxidative Medicine and Cellular Longevity·Omotayo O ErejuwaMohd S Ab Wahab
Dec 3, 2016·Free Radical Biology & Medicine·Barbara TudekElżbieta Speina
May 13, 2017·Journal of Clinical Anesthesia·Ola M ZanatyHeba Abo El Wafa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.