4,6-α-Glucanotransferase activity occurs more widespread in Lactobacillus strains and constitutes a separate GH70 subfamily.

Applied Microbiology and Biotechnology
Hans LeemhuisL Dijkhuizen

Abstract

Family 70 glycoside hydrolase glucansucrase enzymes exclusively occur in lactic acid bacteria and synthesize a wide range of α-D-glucan (abbreviated as α-glucan) oligo- and polysaccharides. Of the 47 characterized GH70 enzymes, 46 use sucrose as glucose donor. A single GH70 enzyme was recently found to be inactive with sucrose and to utilize maltooligosaccharides [(1→4)-α-D-glucooligosaccharides] as glucose donor substrates for α-glucan synthesis, acting as a 4,6-α-glucanotransferase (4,6-αGT) enzyme. Here, we report the characterization of two further GH70 4,6-αGT enzymes, i.e., from Lactobacillus reuteri strains DSM 20016 and ML1, which use maltooligosaccharides as glucose donor. Both enzymes cleave α1→4 glycosidic linkages and add the released glucose moieties one by one to the non-reducing end of growing linear α-glucan chains via α1→6 glycosidic linkages (α1→4 to α1→6 transfer activity). In this way, they convert pure maltooligosaccharide substrates into linear α-glucan product mixtures with about 50% α1→6 glycosidic bonds (isomalto/maltooligosaccharides). These new α-glucan products may provide an exciting type of carbohydrate for the food industry. The results show that 4,6-αGTs occur more widespread in family GH70 and c...Continue Reading

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Citations

Oct 7, 2015·Frontiers in Microbiology·María I TorinoFernanda Mozzi
Jan 15, 2016·The Journal of Biological Chemistry·Marlène VuilleminMagali Remaud-Siméon
Mar 28, 2018·Annual Review of Food Science and Technology·Kieran M LynchElke K Arendt
Jan 1, 2019·Critical Reviews in Food Science and Nutrition·Wei XuWanmeng Mu
May 10, 2021·International Journal of Biological Macromolecules·Dan LiZhiyao Li
Nov 21, 2014·Journal of Agricultural and Food Chemistry·Hans LeemhuisLubbert Dijkhuizen

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Datasets Mentioned

BETA
ABQ83597
AAU08003.1

Methods Mentioned

BETA
PCR
electrophoresis
nuclear magnetic resonance
acetylation
transglycosylation
1
H
glycosylation

Software Mentioned

HPAEC
BLAST
ClustalW2
SignalP

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