4D non-uniformly sampled HCBCACON and ¹J(NCα)-selective HCBCANCO experiments for the sequential assignment and chemical shift analysis of intrinsically disordered proteins.

Journal of Biomolecular NMR
Jiří NováčekVladimír Sklenář

Abstract

A pair of 4D NMR experiments for the backbone assignment of disordered proteins is presented. The experiments exploit (13)C direct detection and non-uniform sampling of the indirectly detected dimensions, and provide correlations of the aliphatic proton (H(α), and H(β)) and carbon (C(α), C(β)) resonance frequencies to the protein backbone. Thus, all the chemical shifts regularly used to map the transient secondary structure motifs in the intrinsically disordered proteins (H(α), C(α), C(β), C', and N) can be extracted from each spectrum. Compared to the commonly used assignment strategy based on matching the C(α) and C(β) chemical shifts, inclusion of the H(α) and H(β) provides up to three extra resonance frequencies that decrease the chance of ambiguous assignment. The experiments were successfully applied to the original assignment of a 12.8 kDa intrinsically disordered protein having a high content of proline residues (26 %) in the sequence.

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Citations

Jan 15, 2013·Journal of Biomolecular NMR·Wolfgang BermelSoraya Serrano
Nov 10, 2013·Journal of Biomolecular NMR·Wolfgang BermelAnna Zawadzka-Kazimierczuk
Apr 5, 2014·Journal of Biomolecular NMR·David Pantoja-Uceda, Jorge Santoro
Feb 20, 2016·Journal of Biomolecular NMR·Alessandro PiaiAnna Zawadzka-Kazimierczuk
Jun 25, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Krzysztof KazimierczukWiktor Koźmiński
Jun 25, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jaka KrageljMalene Ringkjøbing Jensen
Oct 13, 2012·Chembiochem : a European Journal of Chemical Biology·Wolfgang BermelRoberta Pierattelli
Jul 4, 2015·Biomolecular NMR Assignments·Vojtěch KubáňLukáš Žídek
Apr 24, 2015·Journal of Biomolecular NMR·Paweł DziekańskiAnna Zawadzka-Kazimierczuk
Mar 25, 2014·Journal of Magnetic Resonance·Jiří NováčekVladimír Sklenář
Feb 12, 2015·Progress in Nuclear Magnetic Resonance Spectroscopy·Isabella C Felli, Roberta Pierattelli
Mar 25, 2014·Journal of Magnetic Resonance·Isabella C Felli, Roberta Pierattelli
Oct 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·András PerczelJosef Jampílek
Apr 12, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christoph WiedemannRamadurai Ramachandran
Mar 2, 2018·Journal of Biomolecular NMR·Maria Grazia MurraliIsabella C Felli
Dec 12, 2018·The Journal of Physical Chemistry. B·Erik C CookScott A Showalter

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