5-Aminolevulinic acid tumor paint and photodynamic therapy for myxofibrosarcoma: an in vitro study

Journal of Orthopaedic Surgery and Research
Shachar KenanAdam S Levin

Abstract

5-Aminolevulinic acid (5-ALA), a fluorescent contrast agent, has been used for tumor paint and photodynamic therapy (PDT) for various tumors, but its use with soft tissue sarcomas is not well documented. Myxofibrosarcoma, a subtype of soft tissue sarcoma with a high local recurrence rate, may benefit from similar types of treatment. The purpose of this study was to analyze the effects of 5-ALA tumor paint and PDT on a myxofibrosarcoma cell line. Tumor paint was assessed by exposing micromass pellets of human adipose-derived stromal (ADS) cells or myxofibrosarcoma (MUG-Myx1) cells to 5-ALA. Cell pellets were then visualized using a microscope at established excitation and emission wavelengths. Corrected total cell fluorescence was calculated per accepted protocols. Photodynamic therapy was similarly assessed by exposing ADS and MUG-Myx1 cells to 5-ALA, with subsequent analysis via flow cytometry and real-time confocal microscopy. The use of 5-ALA tumor paint led to a selective fluorescence in MUG-Myx1 cells. Findings were confirmed by flow cytometry. Interestingly, flow cytometry results showed progressive selective cell death with increasing 5-ALA exposure as a result of the PDT effect. PDT was further confirmed using confocal ...Continue Reading

References

Apr 1, 1977·Cancer·S W Weiss, F M Enzinger
Nov 1, 1987·British Journal of Cancer·Z Malik, H Lugaci
Apr 1, 1996·The American Journal of Surgical Pathology·T MentzelC D Fletcher
Jun 28, 2006·Clinical Orthopaedics and Related Research·Mark W ManosoEdward A Athanasian
Oct 25, 2007·Deutsche medizinische Wochenschrift·S DenzingerW Otto
Apr 5, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Barbara Krammer, Kristjan Plaetzer
Oct 19, 2010·International Journal of Radiation Oncology, Biology, Physics·Karl E HaglundElizabeth H Baldini
Dec 17, 2014·Journal of Laboratory Automation·Andrew P Aijian, Robin L Garrell
Apr 15, 2016·Experimental Biology and Medicine·Nan-Shan Chang
Aug 9, 2016·Photomedicine and Laser Surgery·Bradley WhitePaige J Baugher
Dec 3, 2017·Photodiagnosis and Photodynamic Therapy·Toshiya TeshigawaraFumitaka Kikkawa
Sep 2, 2018·Photodiagnosis and Photodynamic Therapy·Yusuke MatobaDaisuke Aoki
Apr 17, 2019·Acta neurochirurgica·Michael SchwakeWalter Stummer
Sep 26, 2019·Frontiers in Oncology·Sergey A GoryaynovAlexandr A Potapov

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Methods Mentioned

BETA
flow
confocal microscopy
flow cytometry

Software Mentioned

ImageJ

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