5-Azacytidine and BDNF enhance the maturation of neurons derived from EGF-generated neural stem cells

Experimental Neurology
M Schinstine, L Iacovitti

Abstract

EGF-generated neural stem cells can form astrocytes, neurons, and oligodendrocytes upon differentiation; however, the proportion of cells that actually form neurons is very small. In the present study, we have studied the effect that 5-azacytidine (5AzaC), a demethylation agent, and brain-derived growth factor (BDNF) have on the differentiation and maturation of neurons originating from EGF-generated neural stem cells. Stem cells were maintained under a variety of culture conditions using combinations of 5AzaC and BDNF either alone or together. More neurons, as determined by the number of beta-tubulin III-immunoreactive somata, were present in cultures maintained in BDNF medium (a nearly fourfold increase compared to control cultures). 5AzaC did not significantly affect neuronal number, regardless of the presence of BDNF. In addition to neuronal number, the effect of 5AzaC and BDNF on the distribution of the microtubule proteins MAP2 and Tau was analyzed. In most cultures, MAP2 and Tau were colocalized throughout the neuron. In contrast, neurons cotreated with 5AzaC and BDNF contained neurons that began to exhibit cytoskeletal segregation of MAP2 into the somatodendritic compartments. Tau remained dispersed within the somata an...Continue Reading

References

Aug 21, 1992·Brain Research. Developmental Brain Research·C C Stichel, H W Müller
Jan 15, 1991·Brain Research. Developmental Brain Research·E J KilbourneE L Sabban
Feb 7, 1992·Cell·N S Foulkes, P Sassone-Corsi
Apr 8, 1988·Cell·H Cedar
Sep 25, 1986·Neuroscience Letters·K YoshidaY Tsukada
Oct 1, 1985·The Journal of Cell Biology·L I BinderL I Rebhun
Dec 15, 1982·Journal of Molecular Biology·S M Taylor, P A Jones
Jan 1, 1983·Experimental Brain Research·S A Bayer
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A MatusT Hugh-Jones
Jul 1, 1995·Experimental Neurology·R L Snow, J A Robson
Jul 15, 1994·Brain Research. Developmental Brain Research·H R Widmer, F Hefti
Mar 1, 1993·Experimental Cell Research·D TuilF Phan-Dinh-Tuy

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Citations

Aug 5, 2014·Cellular Reprogramming·Keerat KaurLeonard M Eisenberg
Jul 3, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alinda R Fernandes, Divya M Chari
Aug 18, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Miguel A López-ToledanoEulalia Bazán
Dec 22, 1999·Gene Therapy·F J RubioA Martínez-Serrano
Aug 19, 2004·Journal of Biomedical Science·Yu-Show FuMing-Yuan Min
Jul 20, 2019·In Vitro Cellular & Developmental Biology. Animal·Narawadee RujanapunParinya Noisa

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