PMID: 7512879Jan 1, 1994Paper

5-azacytidine produces differential undercondensation of alpha, beta and classical human satellite DNAs

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
J L FernándezJ Gosálvez

Abstract

Fluorescence in situ hybridization employing human alphoid, beta and classical satellite DNA probes was performed on 5-azacytidine treated and untreated chromosomes obtained from human lymphocytes. The individual used in this study presented a polymorphism of constitutive heterochromatin of chromosomes 1 and 9 as revealed by in situ digestion with the restriction endonuclease Alul. Neither the alphoid nor the beta satellite DNA domains were susceptible to condensation-inhibition by 5-azacytidine. Only the classical satellite localized on chromosome 9 was affected. The constitutive heterochromatin size polymorphism was shown to depend mainly on variations of the classical satellite DNA domain. Therefore, condensation-inhibition, as a phenomenon which may modify the natural folding of the chromatin fibre, regionally affects human constitutive heterochromatin and seems to be dependent on the heterochromatic family.

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Citations

Aug 19, 2000·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·E Pinna-SennJ C Stockert
May 1, 1995·Journal of Medical Genetics·E KanavakisG Adam
Aug 21, 2019·Cells·Andrea Scelfo, Daniele Fachinetti

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